Growing HeLa cells
jkuruvil at lamar.colostate.edu
Tue Jul 16 18:18:39 EST 2002
I think they are an S3 background.
Nick Theodorakis wrote:
> On Mon, 15 Jul 2002 14:35:24 -0600, Jes Kuruvilla
> <jkuruvil at lamar.colostate.edu> wrote:
> >Hi all,
> >Does anybody know how to get He La cells (spinner adapted)
> >to grow successfully? I need to make some nuclear extract. I
> >have tried ( Ca- deficient) Jokliks media with 10% fetal
> >Calf serum and have been keeping the density at 2X 10^5.
> >They still seem to be just sitting around in the flask and
> >not doubling. Most of the cells are found in clumps. Is this
> >usual for cells taken from a trypsinized 10 cm plate??
> Which "strain" of HeLa's are you using? When I was in grad. school, it
> was "lab dogma" that HeLa S3 could grow in spinners (and they did)
> whereas our other strain did poorly in spinners.
> Nick Theodorakis
> nicholas_theodorakis at urmc.rochester.edu
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