Dissociating Cos 7 cells for hanging drop cultures

Lisa A. Fagg l.fagg at ucl.ac.uk
Thu Jun 6 08:29:42 EST 2002


I'm trying to develop a protocol for producing 500 cell spherical (ish)
clusters of transfected cos cells.  At the moment I'm using un-transfected
cells in 75mm2 flasks and dissociating them with 2 min of trypsin and a lot
of banging BUT the cells don't reaggregate very well.  The hanging drops are
10 and 20 µl each - containing 500 dissociated cells.
Help anyone?  Thanks!

-- 
Dr. Lisa A. Fagg
Senior Research Fellow
The Institute of Ophthalmology
University College London
11-43 Bath Street, London EC1V 9EL
Email: l.fagg at ucl.ac.uk  Phone: (0207) 608-4061


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