G418 selection

Biplab Bose biplabbose at hotmail.com
Sat Mar 16 06:10:23 EST 2002


Hi!
Am trying to transform COS1 cells with a mammalian expression vector
carrying neomycine resistance gene. Can any one please tell me what
concentration of G418 is usually used for selection of positive transformed?
And what is the normal time required for G418 to show its action?
thanks


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