Adrenoceptor transfection

Thomas W. Moon TMOON at science.uottawa.ca
Mon Mar 18 16:37:40 EST 2002


Hi:

I'm new at this business of transfection. The literature suggests 
that COS7 cells are good for expression work on beta-
adrenoceptors. We have a beta-AR from rainbow trout that we want 
to express and examine its pharmacology, but we have found that 
COS7 cells have an endogenous beta-AR that respond to 
isoproterenol. Does anyone know a cell line that we can use to 
transfect our fish beta-AR so pharmacology studies?

thanks, Tom
T.W. Moon, PhD
Professor
Department of Biology
University of Ottawa
30 Marie Curie St.,
P.O. Box 450, Stn A
Ottawa, Ontario  K1N 6N5 Canada

TEL: (613)562-5800, ext 6002
FAX:  (613)562-5486
e-mail: tmoon at science.uottawa.ca
http://www.science.uottawa.ca/~biology/profs/tmoon.htm
http://myprofile.cos.com/moont50
visit CSZ at http://www.csz-scz.ca//jpellerin/csz/

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