gotta get those #$@&* cells to STICK!!!
say.no at spam.now
Fri Mar 22 03:07:29 EST 2002
john.hines at yale.edu wrote:
> In article <3C7C9DC9.2CCACD5B at iNOSPAMats.csic.es>, oswaldo
> <oswaldo at iNOSPAMats.csic.es> wrote:
> > Des the PBS that you use contain divalent cations (Ca2+ and Mg2+)?. Ca
> > and Mg-free PBS detaches certain cell types readily.
> > oswaldo
> Well, a lot of cells detach even if I just change the medium, which has
> the full complement of Ca+2 and Mg+2. So I'm guessing that it'll take
> more than that, but perhaps upping the concentration of divalent cations
> in the PBS rinses would minimize the cell loss at least in those steps.
Have tried using laminin coated coverslips or dishes?
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