possible physiological correlates of CB1-transfected AtT20 cells

rsostek at harvardapparatus.com rsostek at harvardapparatus.com
Fri Oct 18 14:25:32 EST 2002


BIORESEARCH TECHNOLOGY UPDATE #2019

This BIORESEARCH TECHNOLOGY UPDATE (BTU) is to inform you about an article
that investigates the possible physiological correlates of CB1-transfected
AtT20 cells having shown a rapid desensitization of these receptors
following activation of protein kinase C by 4-a-phorbol. They have studied
the effects of repeated cannabinoid treatment on ventral tegmental area
dopaminergic neuronal firing in vitro. To accomplish this, the paper used
the Vibroslice , the brain slice chamber PDMI-2 open perfusion
micro-incubator, Co2/pH controller, TC-202 bipolar temperature controller.
Electrode placement was achieved using a remotely controlled DC-3K motorized
micromanipulator, Neurolog a.c. preamplifier, and a peristaltic pump from
Harvard Apparatus Company.

 LACK OF RESPONSE SUPPRESSION FOLLOWS REPEATED VENTRAL TEGMENTAL CANNABINOID
ADMINISTRATION: AN IN VITROELECTROPHYSIOLOGICAL STUDY, J. F. CHEER,* C. A.
MARSDEN, D. A. KENDALL and R. MASON Neuroscience Vol. 99, No. 4, pp.
661-667, 2000
School of Biomedical Sciences, University of Nottingham Medical School,
Queen's Medical Centre, Nottingham NG7 2UH, UK

The information on products utilized in this article: Vibroslice (pg. M73) ,
the brain slice chamber PDMI-2 open perfusion micro-incubator (pg. M55),
TC-202 bipolar temperature controller (pg. M63), Electrode placement was
achieved using a remotely controlled DC-3K motorized micromanipulator
(pg.M104 ), Neurolog a.c. preamplifier (pg.I 128), Co2/pH controller (pg.
M64) and a peristaltic pump (A23) from Harvard Apparatus Company in the
Harvard Apparatus Catalog or at:

For a complete listing of all the cell biology and electrophysiology
products from Harvard Apparatus Company look at:

http://www.harvardapparatus.com/catalog/t_contents1.html or
www.warneronline.com

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information that may enhance your research capabilities. If you have any
requests for specific kinds of information on products or applications
specific to your research, please send your e-mail request to
support at warneronline.com or call 800-599-4203 for ordering or technical
support.








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