cyclines Western blot

Tina anfortinjp at
Thu Jan 9 19:50:06 EST 2003

Is there any special technical tips to do Western Blot (on primary cells
extracts) for cell cycle cyclines ?
Is there a high sensitivity of cyclines to degradation ?
Do cell lysates need to be done with a lysis buffer containing protease
inhibitors followed by addition of Sample buffer or or lysed cells directly
with Sample Buffer?

Thanks a lot!

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