Enzyme-friendly fixative

EK nobody at elnino.com
Sat Feb 14 20:21:43 EST 2004


Just search for protocols on cells and tissue fixation. Ethanol,
paraformaldehyde, formaldehyde, glutaraldehyde, detergents (Triton X-100,
CTAB, ...), organic solvents (toluene, chlorophorm, ...). Specific procedure
would depend what exactly you are studying. If you are doing e.g.
microscopy, that would be either ethanol fixation, or (one of the methods)
2-4% paraformaldehyde followed by brief permeabilization by Triton X-100 in
1-2% sucrose....

-Emir

"Louis Hom" <lhom at OCF.Berkeley.EDU> wrote in message
news:c0l6r1$1gtf$1 at agate.berkeley.edu...
> Can anyone suggest a reagent for fixing cells that will preserve native
> enzyme activity?
> -- 
>
____________________________________________________________________________
__
> Lou Hom >K'93
> lhom at ocf.berkeley.edu
> http://www.ocf.berkeley.edu/~lhom/





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