Resources for choosing a cell line

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Fri Jan 9 11:44:52 EST 2004


Tom Anderson wrote:

> For example, i'd like to do some experiments with epithelial cells;
> ideally, they'd be human, properly polarised, and not too transformed (and
> i don't want to get into primary cultures yet). However, i don't know
> which cell line i should use for this; HeLa is too screwed up, MDCK and
> CHO aren't human, MCF-7 or HEP G2 aren't too bad but are cancer lines, and
> i can't think of any others off the top of my head.

Have you considered CaCo-2? This is a human colon cancer cell line, but
grows in epitelia like assemblies and are used as a standard model for
transport (e.g. blood/brain-barrier). And they are easy to maintain.



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