Resources for choosing a cell line

EK someone at microsoft.net
Fri Jan 9 12:22:33 EST 2004


"Tom Anderson" <ucgatan at socrates-a.ucl.ac.uk> wrote in message
news:Pine.SOL.4.33.0401071344400.6540-100000 at socrates-a.ucl.ac.uk...
> Hi,
>
> Are there any resources out there to help with choosing a cell line for an
> experiment?
>
> For example, i'd like to do some experiments with epithelial cells;
> ideally, they'd be human, properly polarised, and not too transformed (and
> i don't want to get into primary cultures yet). However, i don't know
> which cell line i should use for this; HeLa is too screwed up, MDCK and
> CHO aren't human, MCF-7 or HEP G2 aren't too bad but are cancer lines, and
> i can't think of any others off the top of my head.
>
> The only sources of information i know of that i can turn to are the
> ATCC/ECACC catalogues (which are are focused on the lines rather than the
> applications) and word of mouth and literature searches, both of which are
> a bit haphazard. Any other suggestions?

There is also www.dsmz.de (Deutche Sammlung von Mikroorganismen und
Zellkulturen).

-Emir





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