Resources for choosing a cell line

Pamela Norton pamela.norton at mail.tju.edu
Tue Jan 13 12:55:12 EST 2004


In article <Ae5Mb.57$_4.27923 at news.uchicago.edu>, EK
<someone at microsoft.net> wrote:

> "Tom Anderson" <ucgatan at socrates-a.ucl.ac.uk> wrote in message
> news:Pine.SOL.4.33.0401071344400.6540-100000 at socrates-a.ucl.ac.uk...
> > Hi,
> >
> > Are there any resources out there to help with choosing a cell line for an
> > experiment?
> >
> > For example, i'd like to do some experiments with epithelial cells;
> > ideally, they'd be human, properly polarised, and not too transformed (and
> > i don't want to get into primary cultures yet). However, i don't know
> > which cell line i should use for this; HeLa is too screwed up, MDCK and
> > CHO aren't human, MCF-7 or HEP G2 aren't too bad but are cancer lines, and
> > i can't think of any others off the top of my head.
> >
> > The only sources of information i know of that i can turn to are the
> > ATCC/ECACC catalogues (which are are focused on the lines rather than the
> > applications) and word of mouth and literature searches, both of which are
> > a bit haphazard. Any other suggestions?
> >
> > Thanks,
> > tom
> >
> Why not primary? Have you looked into HUVEC?


HUVECs are human umbilical vein endothelial cells. 

To the original poster, as long as the cells are properly polarized,
perhaps the transformed status is less important?  One possibility
might be to run the same experiment in two different cell lines.



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