3H Thymidine

Nick Theodorakis nick_theodorakis at hotmail.com
Wed Jan 28 22:23:51 EST 2004


On 27 Jan 2004 21:32:11 -0000, farfor444 at netscape.net (Text) wrote:

>Hello All, i have a big problem using 3H Thymidine in cell culture of 
>lymphoblastoid cells. The growth of the cells is very inhibited with the
>thymidine in the medium. this inhibition of growth seems more pronounced 
>when i put more 1 µci/ml of medium Versus 0.2 µci/ml.
>Any explications for this observation? and if its possible, can you give 
>me some references which will help me to understand more this strange 
>world of thymidine :)

What is the final concentration of thymidine in the medium? There is
an old techique for blocking cells called thymidine block, but it
requires millimolar levels of thymidine.

Nick


-- 
Nick Theodorakis
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu 



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