3H Thymidine

Nick Theodorakis nick_theodorakis at hotmail.com
Wed Jan 28 22:23:51 EST 2004

On 27 Jan 2004 21:32:11 -0000, farfor444 at netscape.net (Text) wrote:

>Hello All, i have a big problem using 3H Thymidine in cell culture of 
>lymphoblastoid cells. The growth of the cells is very inhibited with the
>thymidine in the medium. this inhibition of growth seems more pronounced 
>when i put more 1 µci/ml of medium Versus 0.2 µci/ml.
>Any explications for this observation? and if its possible, can you give 
>me some references which will help me to understand more this strange 
>world of thymidine :)

What is the final concentration of thymidine in the medium? There is
an old techique for blocking cells called thymidine block, but it
requires millimolar levels of thymidine.


Nick Theodorakis
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu 

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