clumping of MEFs in suspension

Anette anette.huebner at umassmed.edu
Thu Apr 7 12:33:39 EST 2005


Hi everybody,
I just finished a MEF prep (one of many I have done so far), meaning I 
just plated my cells 2 hours ago. The efficiency of the prep seemed 
great.
When I checked on them 10 min ago, I realized that the cells are 
forming big clusters in suspensions. I have NEVER seen this before.
Well, these are not WT MEFs, but a new mutant. I was wondering whether 
this is something that happens for whatever reason, or, whether it 
could be a phenotype.

I would appreciate any input.
Thanx, Anette

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