[Cell-biology] Re: suggestions for new PAGE apparatus

Jayakumar, R R.Jayakumar at roswellpark.org
Fri Aug 5 12:35:18 EST 2005

Try out the Hoefer website for more details

http://www.hoeferinc.com/static_products.asp?page=54 . The mightly small
gel aparatuses use alumina plates for heat exchange.  I have used this
apparatus couple of years back. The only problem is that the alumina
plates tend to crack very easily and they are costly to replace.  But
they give great resolutions.

best of luck


-----Original Message-----
From: cellbiol-bounces at oat.bio.indiana.edu
[mailto:cellbiol-bounces at oat.bio.indiana.edu] On Behalf Of Dr Engelbert
Sent: Friday, August 05, 2005 4:10 AM
To: cellbiol at magpie.bio.indiana.edu
Subject: [Cell-biology] Re: suggestions for new PAGE apparatus

Dr. James J. Campanella wrote:

> Hi everyone,
> I am in the market to buy a new PAGE electrophoresis set-up. I refuse
> buy Biorad again. I have been burnt by their low quality equipment
> We have begun pouring a large number of gels,

I have been quite satisfied with the Hoefer system obtained from
Pharmacia (now GE, unless that has changed again while I blinked). In
particular their casting boxes for 5 or 10 gels are handy. 

However, the combs are ill-designed and allow air access to the stacking
gels, so the wells do not polymerise completly. In the multicaster boxes
that is no problem, but if you cast gels separately its a PITA. I had
better combs made in our workshop to avoid the problem. 
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