human plasma on cell cultures

Philipp Pagel pDOTpagel at gsf.de
Thu Mar 10 08:17:37 EST 2005


> I am trying to add human plasma (derived from citrate blood) to cell 
> cultures. The set-up is the following: HEK293 cells are cultured in 12 
> well-plates in 1 ml of complete medium (DMEM with serum and 
> antibiotics). Then 700 microliters of human plasma are added (without 
> removing the medium). After 4h the whole stuff is coagulated! Does 
> anybody know how to avoid blood clotting under these circumstances?

How are you preparing the plasma? Are you sure it's not serum? In the
latter case it is not surprising to see coagulation.

cu
	Philipp



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