[Cell-biology] red fluorescent DNA staining

Huseyin Sumer huseyin.sumer at med.monash.edu.au
Wed Sep 14 20:58:12 EST 2005


Dear Jos,

I came across your question on GFP and using a vital red fluorescent dye 
for DNA staining on the web. Did you get anywhere with all this? I am 
also looking into using an alternate to H33342 and GFP, your help will 
be greatly appreciated.

kind regards

Huseyin



_________________________________________________________________

Dear reader,

Currently I am examining  living cells with Green Fluorescent Protein
and I want to combine this with a red fluorescent dye to visualize DNA
in vital cells using confocal laser scanning microscopy. I cannot use
Hoechst or other dyes with UV excitation, since cells will be damaged
and since our laser cannot excite Hoechst. Does anyone know of a vital
red fluorescent DNA dye with absorption spectrum around 567 nm and
emission above 600 nm? I already tried several dyes from Molecular
Probes (Syto-17 and Syto-64) but they appear to stain no nuclear DNA in
living eukaryotic cells.

Thank you very much for your reply.
Jos Broers




-- 
Huseyin Sumer  Ph.D.
NH&MRC Peter Doherty Biomedical Research Fellow
Centre For Reproduction and Development
Monash Institute of Medical Research

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