[Cell-biology] Re: S phase cell marker for immunofluorescence

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Thu Aug 17 08:59:18 EST 2006


Collynn Woeller wrote:

>  I am looking for a marker for S phase cells. Is there any marker I could
> use to determine which cells are in S phase when looking at my cells on
> slides (for immunofluorescence preferably? I am using MCF-7 cells.


S phase is characterised by DNA doubling, so any DNA stain can be used
as long as you can quantify it. This includes the fluorescent nuclear
dyes like propidium iodide or Hoechst and the non-fluorescent Feulgen
staining. Quantitation by fluorescence is easy (FACS, image processing
microscopy), Feulgen can be quantified by a microscope-linked
photometer, specialised equipment that is probably rare nowadays. The
technique however was extensively used 20, 30 years ago for life cycle
investigations (determining which are the haploid and diploid forms).


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