[Cell-biology] Re: Cytoplasmic marker enzyme (Plant)

[Dr Engelbert Buxbaum]

Jana Hakenjos wrote:

> Dear all,
> 
> I am isolating chloroplasts from Physcomitrella patens (a moss) via a
> Percoll density gradient. To test for contaminations from other
> compartments I want to measure enzyme activities. But I can't find a
> good candidate for the cytoplasm that fulfils following "requirements":

Glycolysis (Embden-Meyerhoff-pathway) occurs in the cytoplasm, G3PDH is
NAD-dependent and would make an obvious candidate.