[Cell-biology] Re: Cytoplasmic marker enzyme (Plant)

Dr Engelbert Buxbaum via cellbiol%40net.bio.net (by engelbert_buxbaum At hotmail.com)
Sun Dec 10 03:36:24 EST 2006


Jana Hakenjos wrote:

> Dear all,
> 
> I am isolating chloroplasts from Physcomitrella patens (a moss) via a
> Percoll density gradient. To test for contaminations from other
> compartments I want to measure enzyme activities. But I can't find a
> good candidate for the cytoplasm that fulfils following "requirements":

Glycolysis (Embden-Meyerhoff-pathway) occurs in the cytoplasm, G3PDH is
NAD-dependent and would make an obvious candidate. 


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