[Cell-biology] Soft agar cloning of Tumor cells

Ken Garson kgarson at uottawa.ca
Thu Jan 12 10:23:57 EST 2006


Hi,

I am screening primary tumor cells for their ability to grow in soft agar.
 I have recovered adherent cell lines by sucking out the colonies with a
pipette and then mechanically breaking up the agar/cell plug to release
the tumor cells.  The mechanical disruption is not very efficient.  Is
there a method for recovering colonies from soft agar and generating a
nice single cell suspension of viable cells?

Thanks!

Ken



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