[Cell-biology] Soft agar cloning of Tumor cells
kgarson at uottawa.ca
Thu Jan 12 10:23:57 EST 2006
I am screening primary tumor cells for their ability to grow in soft agar.
I have recovered adherent cell lines by sucking out the colonies with a
pipette and then mechanically breaking up the agar/cell plug to release
the tumor cells. The mechanical disruption is not very efficient. Is
there a method for recovering colonies from soft agar and generating a
nice single cell suspension of viable cells?
More information about the Cellbiol