[Cell-biology] Re: Soft agar cloning of Tumor cells

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Tue Jan 17 05:13:53 EST 2006


Ken Garson wrote:

> 
> Hi,
> 
> I am screening primary tumor cells for their ability to grow in soft agar.
>  I have recovered adherent cell lines by sucking out the colonies with a
> pipette and then mechanically breaking up the agar/cell plug to release
> the tumor cells.  The mechanical disruption is not very efficient.  

Have you tried to place the agar pieces into cell culture dishes with
medium and waiting for the cells to grow out (that is, without
disruption of the agar piece)? 


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