[Cell-biology] PMA activation of cells

Anshul awaan686 at student.otago.ac.nz
Thu Jul 6 17:58:54 EST 2006

Hi all,

I am interested in activation U251 cells with PMA and then check the 
expression levels of a particular protein using RT-PCR. I went through a 
few papers to get a protocol and found that cells were grown in complete 
media till 70% confluence and then cells were grown in a serm free media 
for 16-18 hrs prior to PMA activation. However I also found a few papers 
in which they have not used any serum free media and directly activated 
the cells using PMA.

Now can anyone tell me what can be real use of growing cells in serum 
free media and also if this step is necessary or not?


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