[Cell-biology] DNA denaturing agents

shankar via cellbiol%40net.bio.net (by shankargan2006 At gmail.com)
Sun Nov 12 02:36:53 EST 2006


Hi everybody,

What pH and salt concentration is ideal for denaturing a double
stranded Lambda DNA into a single strand and also compatible with the
streptavidin-biotin bonds

Can Lambda exo be used along with SSB, to digest the double stranded
Lambda DNA and also preserving the single strand by means of SSB? Has
anyone done some experiments using both Lambda exo and SSB together to
denature the Lambda DNA? If so, what are the conditions that have been
tried to get a single strand?


Looking forward to yours response..



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