[Cell-biology] Western blot detection of membrane proteins

Samuel C McNeely via cellbiol%40net.bio.net (by scmcne01 from louisville.edu)
Mon Mar 5 11:07:38 EST 2007


I need to detect MRP1, a membrane protein, by western blot and have been unable to do so. Do membrane proteins require higher concentrations of detergent in order to be soluble in lysis solution? What is the ideal lysis buffer? Thanks.


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