[Cell-biology] EB formation
(by khardah from hotmail.com)
Wed May 23 18:12:47 EST 2007
I am trying to differentiate mouse ES cells to cardiomyocytes by using
EB formation but was not able to get any cardiomyocytes. Does anybody
have any protocol for this? I am using IMDM media with 15% FBS,
pen/strp and beta-ME. Should I use MTG insteadd of beta-ME? Your help
will be highly appreciated.
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