[Cell-biology] Water Crystals in Frozen Bacteria!

tarun gupta via cellbiol%40net.bio.net (by hotbacteria from rediffmail.com)
Wed Oct 3 15:33:20 EST 2007


  
Dear Friends, 

I have a small query of scientific nature. 

I remember once my mom made milk based ice cream for me and kept it at 4 degrees in refrigerator. The next thing I found was that ice cream was not smooth and there were water crystals formed. 

Now, my query has two parts:

1. Biological Cells have a large volume of water as its consituents. I have been wondering that when we store our bacterial plates in frozen condition at -80 degrees, don't this water forms sharp crystals and damage the cells? 

If crystals do form, they should damage cellular membranes and macromolecules like proteins and DNA (suspended in solvent, ain't they?)! Logically, this should happen, shouldn't it? 

2. If this does happen, how do these frozen bacteria revive when we bring them back to normal temperatures in appropriate media? Any comments ?

You can see the original entry at Scientific Information Sharing Resource: http://sisr.blogspot.com/2007/10/water-crystal-in-frozen-bacteria.html

Kindly submit your comments at Exciting Science forum: 
http://groups.google.com/group/exciting-science 
or write to me at: hotbacteria [at] gmail [dot] com 

Kind Regards,
Tarun Gupta 

-- 
MSc Human Genomics 
National Centre of Human Genome Studies and Research
Panjab University
Chandigarh-India 

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MSc Human Genomics 
National Centre of Human Genome Studies and Research
Panjab University
Chandigarh-India 
Mob: +91-9888237906

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~Exciting Science forum: http://groups.google.com/group/exciting-science
~Bioinformatics Resources: http://hotbacteria.blogspot.com
~Scientific Information Sharing Resource: http://sisr.blogspot.com
~About Me: http://hotbacteria.tripod.com

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