From stephen.fox from insightbb.com Wed Apr 2 22:21:27 2008 From: stephen.fox from insightbb.com (stephen.fox@insightbb.com) Date: Wed Apr 2 22:37:08 2008 Subject: [Cell-biology] Planarians, RNA, memory, cannibalism & knowlege Message-ID: <5f0d3331e7974d86a52b77c23aa745f8@insightbb.com> I found your response to a forum request for information on memory transfer after seeing a comment on such experiments in Maxwell's book on the evolution of sex, "The Sex Imperative." I believe the copyright on the book is from the early nineties. I also recall hearing just last year that there had been some sort of a reappraisal of Lamarck due to increased understanding of the role of RNA in heritable responses to environmental stimulus. If you have any additional information or thoughts on the Planarian experiments in memory transfer, or, on the current understanding of heritable conditioning I would appreciate your sharing them with me. Perhaps there is a particular resource you could point out to me? Best Regards, Stephen Fox From scimedweb from mail.com Wed Apr 9 23:56:59 2008 From: scimedweb from mail.com (scimedweb@mail.com) Date: Thu Apr 10 09:47:49 2008 Subject: [Cell-biology] Detection, markers and prognostic/predictive significance of disseminated tumor cells. Message-ID: <78c83c63-3a9b-414b-ab8b-7d66c2842b3e@59g2000hsb.googlegroups.com> Disseminated tumor cells: detection, markers and prognostic/predictive significance. by Marc Lacroix InTextoResearch, Baelen, Wallonia, Belgium in Cancer Metastases Research, Nova Sciences Publishers, New York, ISBN 978-1-60456-130-2 (2008), pp. 1-45. The development of distant metastases is the major cause of death from most cancers. In order to predict and prevent tumour spreading, many attempts are being made to detect small numbers of tumour cells that have shed from the primary lesions and have moved to lymph nodes, blood or bone marrow ("disseminated tumor cells", or DTC). This chapter presents the advantages and limitations of techniques used for disseminated tumour cells (DTC) detection. DTC markers used in bladder, breast, cervical, colorectal, esophageal, gastric, head and neck, liver, lung, melanoma, ovarian, pancreatic, prostate, renal, and thyroid cancers are listed. The significance of DTC for prognosis and prediction of response to therapy is examined, with a specific focus on breast cancer. DTC viability and the notion of cell dormancy are also discussed. Markers used to detect DTC from various cancers by RT-PCR: AFP Alpha-fetoprotein Type(s) of cancer: liver AGR2 Anterior gradient homolog 2 (Xenopus laevis) Other name(s): Secreted cement gland protein XAG-2 homolog (hAG-2) ; XAG-2 homolog long protein (HPC8); GOB-4 Type(s) of cancer: breast, colorectal, prostate ANKRD30A Ankyrin repeat domain 30A Other name(s): Breast cancer antigen NY-BR-1; B726P Type(s) of cancer: breast AR Androgen receptor Type(s) of cancer: prostate B305D Antigen B305D Other name(s): B305D, isoform A (B305D-A) B305D, isoform C (B305D-C) Type(s) of cancer: breast B4GALNT1 Beta-1,4-N-acetyl-galactosaminyl transferase 1 Other name(s): UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)- galactosylglucosylceramide N-acetylgalactosaminyltransferase Type(s) of cancer: melanoma CA9 Carbonic anhydrase IX Other name(s): Renal cell carcinoma associated antigen G250 Type(s) of cancer: kidney CALCA Calcitonin/calcitonin-related polypeptide, alpha Type(s) of cancer: thyroid CD44 Antigen CD44 Other name(s): Hermes antigen; PGP1 Type(s) of cancer: breast, head & neck CDH1 Cadherin-1 (epithelial) Other name(s): E-cadherin; uvomorulin Type(s) of cancer: breast CDH6 Cadherin 6, type 2, K-cadherin (fetal kidney) Type(s) of cancer: kidney CEACAM5 Carcino-embryonic antigen-related cell adhesion molecule 5 Other name(s): Carcino-embryonic antigen (CEA) Type(s) of cancer: breast, colorectal, esophagus, head & neck, lung, pancreas, stomach, thyroid CEACAM7 Carcino-embryonic antigen-related cell adhesion molecule 7 Other name(s): Carcinoembryonic antigen 2 (CGM2) Type(s) of cancer: colorectal CGB Chorionic gonadotropin, beta polypeptide Other name(s): ?-human chorionic gonadotropin (?-HCG) Type(s) of cancer: breast CTSD Cathepsin-D Type(s) of cancer: breast, kidney CXCR4 Chemokine, CXC motif, receptor 4 Other name(s): Neuropeptide Y receptor Y3 (NPY3R); lipopolysaccharide- associated protein 3 (LAP3); fusin Type(s) of cancer: breast DCT Dopachrome tautomerase Other name(s): Tyrosinase-related-protein-2 (TYRP2); dopachrome delta- isomerase Type(s) of cancer: melanoma DDC Dopa decarboxylase (aromatic L-amino acid decarboxylase) Type(s) of cancer: prostate, stomach EGFR Epidermal growth factor receptor Other name(s): Oncogene ERBB1; c-erbB-1 Type(s) of cancer: bladder, breast, cervix, colorectal, head & neck, lung, prostate ERBB2 V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/ glioblastoma derived oncogene homolog (avian) (ERBB2) Other name(s): c-erbB-2,; HER-2/neu Type(s) of cancer: breast FABP1 Fatty acid binding protein 1, liver Other name(s): Liver fatty acid binding protein (L-FABP) Type(s) of cancer: breast, colorectal, prostate FOLH1 Folate hydrolase Other name(s): Prostate-specific membrane antigen (PSMA) Type(s) of cancer: prostate GABRP Gamma-aminobutyric acid type A receptor pi (p) subunit Other name(s): GABA receptor A, pi (p) polypeptide (GABARAP); GABA-A receptor, pi (p) polypeptide (GABA A(p)) Type(s) of cancer: breast GALNT6 UDP-N-acetyl-D-galactosamine:polypeptide Other name(s): N-acetylgalactosaminyltransferase 6 ppGalNac-T(6); ? -1-4-N-acetylgalactosaminyl transferase 6; GalNAc transferase 6; (GalNAcT6) Type(s) of cancer: breast GRP Gastrin-releasing peptide Type(s) of cancer: lung, thyroid KLK2 Kallikrein-related peptidase 2 Type(s) of cancer: prostate KLK3 Kallikrein-related peptidase 3 Other name(s): Prostate specific antigen Type(s) of cancer: prostate KLK6 Kallikrein-related peptidase 6 Other name(s): Protease M; neurosin Type(s) of cancer: colorectal, ovary KRT7 Keratin 7 Other name(s): Cytokeratin 7 (CK7); sarcolectin (SCL) Type(s) of cancer: breast, esophagus, lung, ovary KRT8 Keratin 8 Other name(s): Cytokeratin 8 (CK8) Type(s) of cancer: breast KRT18 Keratin 18 Other name(s): Cytokeratin 18 (CK18) Type(s) of cancer: bladder, breast, esophagus KRT19 Keratin 19 Other name(s): Cytokeratin 19 (CK19) Type(s) of cancer: bladder, breast, cervical, colorectal, esophagus, head & neck, lung, ovary, pancreas, stomach, thyroid KRT20 Keratin 20 Other name(s): Cytokeratin 20 (CK20) Type(s) of cancer: bladder, breast, cervical, colorectal, esophagus, head & neck, lung, ovary, pancreas, stomach, thyroid MAGEA1 Melanoma antigen family A, 1 Other name(s): Mage1 Type(s) of cancer: liver MAGEA2 Melanoma antigen family A, 2 Other name(s): Mage2 Type(s) of cancer: lung MAGEA3 Melanoma antigen family A, 3 Other name(s): Mage3 Type(s) of cancer: breast, liver, lung, melanoma MAGEA4 Melanoma antigen family A, 4 Other name(s): Mage4 Type(s) of cancer: lung MAGEA6 Melanoma antigen family A, 6 Other name(s): Mage6 Type(s) of cancer: lung MAGEA12 Melanoma antigen family A, 12 Other name(s): Mage12 Type(s) of cancer: lung MET Protooncogene Met Other name(s): Hepatocyte growth factor receptor (HGFR); renal cell carcinoma, papillary 2 (RCCP2) Type(s) of cancer: breast, stomach MFI2 Antigen p97 Other name(s): Melanotransferrin Type(s) of cancer: melanoma MITF Microphthalmia-associated transcription factor Type(s) of cancer: melanoma MLANA Melan-A Other name(s): Melanoma antigen MART-1 Type(s) of cancer: melanoma MUC1 Mucin-1, transmembrane Other name(s): Carcinoma antigen 15.3 (CA15.3);carcinoma antigen 27.29 (CA27.29); CD227 antigen; episialin; epithelial membrane antigen (EMA); polymorphic epithelial mucin (PEM); peanut-reactive urinary mucin (PUM); tumor-associated glycoprotein 12 (TAG12) Type(s) of cancer: breast, colorectal, lung, stomach MUC2 Mucin 2, oligomeric mucus/gel-forming Type(s) of cancer: breast, colorectal MUC5B Mucin 5B, oligomeric mucus/gel-forming Type(s) of cancer: breast MUC7 Mucin 7, secreted Type(s) of cancer: bladder, breast OR51E2 Olfactory receptor, family 51, subfamily E, member 2 Other name(s): Prostate-specific G protein-coupled receptor (PSGR) Type(s) of cancer: prostate OSBP2 Oxysterol binding protein 2 Type(s) of cancer: colorectal PAX3 Paired box gene 3 Type(s) of cancer: melanoma PIP Prolactin-induced protein Other name(s): Gross cystic disease fluid protein (GCDFP-15) Type(s) of cancer: breast PLAUR Plasminogen activator receptor, urokinase-type Other name(s): Urokinase receptor (uPAR); CD87 antigen Type(s) of cancer: breast PSCA Prostate stem cell antigen Type(s) of cancer: prostate, stomach PTHLH Parathyroid hormone-like hormone Other name(s): PTHrP (parathyroid hormone-related protein) Type(s) of cancer: breast, head & neck S100A14 S100 calcium binding protein A14 Type(s) of cancer: breast, colorectal, prostate S100A16 S100 calcium binding protein A16 Other name(s): S100F; aging-associated protein 13 (AAG13) Type(s) of cancer: breast, colorectal, prostate SBEM Small breast epithelial mucin Other name(s): BS106; B511S Type(s) of cancer: breast SCGB1D2 Secretoglobin family 1D member 2 (Prostatein-like) Other name(s): Lipophilin B (LPB, LPHB); antigen BU101 Type(s) of cancer: breast SCGB2A1 Secretoglobin family 2A, member 1 Other name(s): Mammaglobin 2 (MGB2); mammaglobin B (MGBB); lacryglobin (LGB); lipophilin C (LPC, LPHC) Type(s) of cancer: breast SCGB2A2 Secretoglobin family 2A, member 2 Other name(s): Mammaglobin (MGB); mammaglobin 1 (MGB1); mammaglobin A (MGBA) Type(s) of cancer: breast SERPINB3 Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3 Other name(s): Squamous cell carcinoma antigen 1 (SCCA1) Type(s) of cancer: esophagus, head & neck SERPINB4 Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4 Other name(s): Squamous cell carcinoma antigen 2 (SCCA2) Type(s) of cancer: esophagus, head & neck SERPINB5 Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 Other name(s): Mammary serine protease inhibitor (Maspin) Type(s) of cancer: breast SILV Silver homolog (mouse) Other name(s): 95 kD melanocyte-specific secreted glycoprotein; Pmel17; Gp100 Type(s) of cancer: melanoma SPDEF SAM pointed domain containing ets transcription factor Other name(s): Prostate epithelium-specific Ets transcription factor (PDEF) Type(s) of cancer: breast TACSTD1 Tumor-associated calcium signal transducer 1 Other name(s): Colorectal carcinoma antigen CO17-1A; epithelial glycoprotein 2 (EGP2); epithelial glycoprotein 40kD (EGP40); epithelial cell adhesion molecule (EpCAM); epithelial-specific antigen (ESA); gastrointestinal tumour associated antigen 733-2 (GA733-2); KS1/4 antigen; KSA antigen; membrane component, chromosome 4, surface marker 1 (M4S1); MK-1 antigen; MIC18 antigen; TROP-1 antigen Type(s) of cancer: breast, colorectal, esophagus, lung TERT Telomerase reverse transcriptase Other name(s): Telomerase catalytic subunit Type(s) of cancer: breast, colorectal, liver, melanoma, stomach, thyroid TFF1 Trefoil factor 1 Other name(s): Breast cancer estrogen-inducible sequence (BCEI); gastrointestinal trefoil protein (GTF);pS2 protein Type(s) of cancer: breast TFF3 Trefoil factor 3 Other name(s): Intestinal trefoil factor (ITF);p1.B Type(s) of cancer: breast, colorectal TG Thyroglobulin Type(s) of cancer: thyroid TPO Thyroid peroxidase Type(s) of cancer: thyroid TSHR Thyroid stimulating hormone receptor Type(s) of cancer: thyroid TSPAN8 Tetraspanin 8 Other name(s): Transmembrane 4 superfamily member 3 (TM4SF3); CO-029 Type(s) of cancer: colorectal TYR Tyrosinase Oculocutaneous albinism IA Type(s) of cancer: melanoma TYRP1 Tyrosinase-related protein 1 Other name(s): Melanoma antigen gp75 Type(s) of cancer: melanoma UCHL1 Ubiquitin carboxyl-terminal esterase L1 Other name(s): Ubiquitin thiolesterase; protein gene product 9.5 (PGP 9.5) Type(s) of cancer: lung UPK1A Uroplakin 1A Type(s) of cancer: bladder UPK1B Uroplakin 1B Type(s) of cancer: bladder UPK2 Uroplakin 2 Type(s) of cancer: bladder UPK3 Uroplakin 3 Type(s) of cancer: bladder VIL1 Villin 1 Type(s) of cancer: colorectal From sticher from bioc.unizh.ch Fri Apr 18 08:04:36 2008 From: sticher from bioc.unizh.ch (Patrick Sticher) Date: Fri Apr 18 11:53:25 2008 Subject: [Cell-biology] 6th International NCCR Symposium on New Trends in Structural Biology Message-ID: <48089C64.1080105@bioc.unizh.ch> Dear colleagues, please be informed that the registration slot for the 6th International NCCR Symposium on New Trends in Structural Biology 8 + 9 September 2008, University of Z?rich, Lecture Hall KOH-B10, Z?rich, Switzerland is now open. Online registration is possible directly from the symposium website: www.structuralbiology.uzh.ch/symposium2008.asp where you will also find further information about this event. Confirmed plenary lecturer to date: Stephen C. Kowalczykowski, Keiichi Namba, Poul Nissen, Andrej Sali, Titia Sixma, Jeffrey Skolnick, A. Joshua Wand Please do not hesitate to contact me anytime if you need further information (sticher@bioc.uzh.ch). With best regards, Patrick Sticher The NCCR Structural Biology is a research initiative of the Swiss Science Foundation. Its research encompasses the fields of recombinant protein technologies, macromolecular structure determination and computational biomolecular sciences with a special focus on membrane proteins and supramolecular assemblies/interactions. 19 research groups from Swiss Universities and Research Institutions participate in this network. www.structuralbiology.uzh.ch/ _________________________________ Dr. Patrick Sticher Moser NCCR Scientific Officer Institute of Biochemistry University of Z?rich Winterthurerstrasse 190 CH - 8057 Z?rich Phone +41 / (0)44 / 635 54 84 Fax +41 / (0)44 / 635 59 08 From sticher from bioc.unizh.ch Fri Apr 25 09:28:08 2008 From: sticher from bioc.unizh.ch (Patrick Sticher) Date: Fri Apr 25 10:31:56 2008 Subject: [Cell-biology] First Announcement - Practical Course in 2D Membrane Protein Crystallization and Observation Message-ID: <4811EA78.7000902@bioc.unizh.ch> Dear colleagues, I would like to inform you about following course: 7th NCCR Practical Course and EMBN Summer School PRACTICAL COURSE IN 2D MEMBRANE PROTEIN CRYSTALLIZATION AND OBSERVATION October 20 - 24, 2008, Basel Switzerland Topics include membrane protein expression, solubilization and purification | detergent properties and interactions with lipids and proteins | functional analysis of membrane proteins | 2D-crystallization of membrane proteins | electron microscopy of 2D crystals | image processing | application of other visualization techniques: AFM, IR-spectroscopy, solid-state NMR | The course is directed primarily to PhD students and postdocs with some previous experience in the structural biology of membrane proteins or related fields. The number of participants will be limited to 25. This course is supported by the European Union Marie Curie Conferences and Training Courses Program, and the Swiss NCCR Structural Biology. Junior scientists wishing to attend this course may apply for an EU Marie Curie Fellowship covering course fees and accommodation costs, and providing support towards travel costs. For further information, please see to the course website www.structuralbiology.uzh.ch/membranecourse2008.asp Online registration will be possible end of May. With best regards, Patrick Sticher _________________________________ Dr. Patrick Sticher Moser NCCR Scientific Officer Institute of Biochemistry University of Z?rich Winterthurerstrasse 190 CH - 8057 Z?rich Phone +41 / (0)44 / 635 54 84 Fax +41 / (0)44 / 635 59 08 Mail sticher@bioc.uzh.ch