[Cell-biology] Re: permeabilize living cells
(by nick.theodorakis from gmail.com)
Fri May 23 08:50:53 EST 2008
On May 21, 5:27 pm, "assa sittner" <sitt... from lkb.ens.fr> wrote:
> Hi there,
> I am looking for a reagent that would allow me to weakly permeabilize, or
> just weaken cell membranes in a living cell culture, without killing them,
> in order to facilitate microinjection.
> does anyone know such a reagent (most likely a mild surfactant)?
I've seen a couple of references where people have used low
concentrations of mild detergents, but cells usually hate it when you
make then leaky.
If you are injecting regular cultured cells with a glass
microcapillary there should be no reason to make the membrane weaker;
the capillary will go right through.
nick_theodorakis from hotmail.com
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