From scimedweb from mail.com Thu Apr 2 10:58:13 2009 From: scimedweb from mail.com (scimedweb@mail.com) Date: Thu Apr 2 11:30:00 2009 Subject: [Cell-biology] HUGO standard gene nomenclature is poorly used in clinical studies. Message-ID: <68220869-92f1-42e5-8a74-f4c828af1feb@r37g2000yqn.googlegroups.com> Poor usage of HUGO standard gene nomenclature in breast cancer studies. by Marc Lacroix InTextoResearch, Baelen, Wallonia, Belgium in Breast Cancer Research and Treatment (2009) 114, 385-386 Since 1989, the Human Genome Organisation (HUGO) attempts to ensure that for each gene there is one name and one symbol. The resulting standard nomenclature is, however, poorly applied in clinical studies, which impairs the efficient retrieval of information. This lack of support is reflected in the present survey of 92 articles reporting on disseminated breast cancer cell detection. Representative markers: KRT19 (Keratin 19), more frequently used aliases: cytokeratin 19; cytokeratin-19; CK19; CK-19; SCGB2A2 (Secretoglobin family 2A, member 2), more frequently used aliases: mammaglobin; mammaglobin A; mammaglobin 1; MAM; mam; hMAM; hMAM-A; MG; MGB1; MMG; SERPINB5 (Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5), more frequently used aliases: maspin; MAS; TACSTD1 (Tumor-associated calcium signal transducer 1), more frequently used aliases: epithelial glycoprotein 2; epithelial glycoprotein 40; EGP40; EGP2; KS1/4; GA733-2; From manolia from stanford.edu Thu Apr 9 00:13:37 2009 From: manolia from stanford.edu (Manolia Margaris) Date: Thu Apr 9 07:55:25 2009 Subject: [Cell-biology] ISBMR 9th Course: Biophysics and Structure, Erice-Sicily, 22 Jun-2 Jul '09 Message-ID: <746d7b95-f31e-44eb-8be9-086402480070@v15g2000yqn.googlegroups.com> We like to bring to your attention the 9th Biophysics and Structure Course of the Int'l School of Biological Magnetic Resonance (ISBMR). We kindly ask you share this information with graduate students, postdoctoral fellows, and other colleagues and interested individuals, particularly in the areas of Structural Biology, NMR, Biophysics, Crystallography, Computational Biology, and Biochemistry. Details follow: COURSE TITLE: ISBMR 9th Course: Biophysics and Structure WHEN: 22 June - 2 July 2009 WHERE: Ettore Majorana Foundation and Centre for Scientific Culture, Erice-Sicily, Italy. URL: http://smrl.stanford.edu/erice2009/ Apply online: http://www.stanford.edu/~manolia/erice2009app.fb * COURSE FORMAT and PURPOSE: The format is an Advanced Study Institute (ASI). This course will provide an overview of physical and structural methods in biology and will present technologies related to pathogen detection and treatment using these methods. Both, basic principles and concrete examples will be presented in a long course format. It is an ideal course for graduate students and postdoctoral fellows. Please note that the structure of the course provides a great opportunity for young scientists to present their work. * TOPICS Include: Principles of NMR spectroscopy; Principles of X-ray diffraction; Single-molecule methods; Cryoelectron Microscopy; Molecular dynamics and other computational approaches; Mass spectrometry; Use of NMR data to calculate protein and nucleic acid structures; New RNA and protein NMR methods; Viral pathogens; Bacterial pathogens; Antivirals; Ribosome structure; Translational mechanism and antibiotics; New technologies to study proteins; Solid state NMR; Peptides as drugs; Membrane proteins as drug targets; Structure-based drug design in biotechnology; NMR and physical studies of pathogenic proteins; Coupling genomics and structural studies. APPLICATION PROCEDURE: Applications are now accepted online: http://www.stanford.edu/~manolia/erice2009app.fb Thank you in advance for your time and consideration. From jflood from chem.utoronto.ca Mon Apr 13 14:01:45 2009 From: jflood from chem.utoronto.ca (jflood@chem.utoronto.ca) Date: Mon Apr 13 14:44:06 2009 Subject: [Cell-biology] Fwd: Phe Auxotrophic E. Coli BL21(DE3) Message-ID: <20090413150145.vs0zoqryecoc0kc0@mail.chem.utoronto.ca> Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be used with plasmids with T7 promoter. Preferably BL21 (DE3). Can anybody direct me to a bank, lab or were to buy it? Thnxs From beth.millerman from stiefel.com Tue Apr 14 16:11:36 2009 From: beth.millerman from stiefel.com (Beth Millerman) Date: Tue Apr 14 19:22:59 2009 Subject: [Cell-biology] Procollagen antibody Message-ID: Did you ever find a distributor for anti-rat Pro collagen Type1? I am looking for a vendor and can't seem to find one. Thanks, Beth Millerman/SWC Senior Research Associate Stiefel Laboratories, Inc Direct Dial; 650-739-2982 Internal Dial: 1-2982 Fax: 650-843-2898 From Maja.Jazvinscak.Jembrek from irb.hr Thu Apr 16 05:22:15 2009 From: Maja.Jazvinscak.Jembrek from irb.hr (Maja =?utf-8?b?SmF6dmluxaHEh2FrwqBKZW1icmVr?=) Date: Thu Apr 16 10:43:58 2009 Subject: [Cell-biology] p19 cell culture Message-ID: <20090416122215.13901lphynt7c6hc@webmail.irb.hr> Dear Fabrice, I have found on the WEB one of your old message about the 19 culture cells. Now, I am starting with them, and I have a lot of problems. Do you have some tested protocols or practical advices how to differentiate these cells to neurons? Thank you in advance Maja Jazvinscak Jembrek, PhD Research Associate Laboratory for Molecular Neuropharmacology Division of Molecular Medicine Rudjer Boskovic Institute Bijenicka 54 10000 Zagreb, Croatia Phone/Fax:+385-1-4571268 Email: jazvin@irb.hr From imperat from hotmail.com Mon Apr 20 11:29:37 2009 From: imperat from hotmail.com (imperat@hotmail.com) Date: Mon Apr 20 14:08:27 2009 Subject: [Cell-biology] Primary mouse myoblast culture Message-ID: <5bed84fa-cfff-4750-90e9-4a179a1073cb@g20g2000vba.googlegroups.com> Hi all, I'm in the process of growing primary myoblasts isolated from lower limb muscles, and repeatedly run into problems. Specifically, after a couple of months of successful work, it becomes increasingly difficult to maintain a line in any particular incubator, such that even freshly isolated myoblasts tend to gradually die. Is there any precedence and/ or solution to this problem? I am growing them, on Matrigel-coated plates, under the following conditions: -- 20% FBS -- 10% horse serum (HyClone) -- hi-glucose DMEM -- 10ng/ml bFGF -- 5% CO2 Thanks in advance, Don From sfisher from Exchange.FULLERTON.EDU Wed Apr 22 18:27:37 2009 From: sfisher from Exchange.FULLERTON.EDU (Fisher, Susan) Date: Wed Apr 22 18:58:38 2009 Subject: [Cell-biology] Mycoplasma Infection in Tissue Culture Message-ID: <5DE82FD1891B364890EB6A416F5254579CA95DEC30@SFEXCH4.AD.FULLERTON.EDU> Dear Dr. McKay, I just read your 1998 message regarding a mycoplasma infection. You asked if you could have infected your tissue culture. I am now curious - could the mycoplasma infection in the tissue culture have infected you? I look forward to your response. Sue Fisher Radiation & Biosafety Officer California State University, Fullerton 800 N. State College Blvd., Fullerton, CA 92831 PH: 714-278-2507 It's spring cleaning time again and the safety office can lend a helping hand. Visit http://ehis.fullerton.edu/Publications/EHISWaste.pdf to learn how to properly dispose of hazardous and non-hazardous materials in your workplace. ________________________________ Attention: Cal State Fullerton's area code is changing soon! Beginning Saturday, May 30, 2009, all Fullerton and Irvine campus phone and fax numbers will begin with 657-278 followed by the current extension number. From R.Jayakumar from roswellpark.org Thu Apr 23 08:19:36 2009 From: R.Jayakumar from roswellpark.org (Jayakumar, R) Date: Thu Apr 23 10:20:59 2009 Subject: [Cell-biology] RE: Mycoplasma Infection in Tissue Culture In-Reply-To: <5DE82FD1891B364890EB6A416F5254579CA95DEC30@SFEXCH4.AD.FULLERTON.EDU> References: <5DE82FD1891B364890EB6A416F5254579CA95DEC30@SFEXCH4.AD.FULLERTON.EDU> Message-ID: It is my understanding that myecolplasma freely exist on our skin surface and they tend to infect cell cultures while handling them with dirty hands or gloves. I haven't heard of a case where the reverse is true, simply because we are NOT immunocompromised creatures. Jay -----Original Message----- From: cellbiol-bounces@oat.bio.indiana.edu [mailto:cellbiol-bounces@oat.bio.indiana.edu] On Behalf Of Fisher, Susan Sent: Wednesday, April 22, 2009 7:28 PM To: 'cellbiol@iubio.bio.indiana.edu' Subject: [Cell-biology] Mycoplasma Infection in Tissue Culture Dear Dr. McKay, I just read your 1998 message regarding a mycoplasma infection. You asked if you could have infected your tissue culture. I am now curious - could the mycoplasma infection in the tissue culture have infected you? I look forward to your response. Sue Fisher Radiation & Biosafety Officer California State University, Fullerton 800 N. State College Blvd., Fullerton, CA 92831 PH: 714-278-2507 It's spring cleaning time again and the safety office can lend a helping hand. Visit http://ehis.fullerton.edu/Publications/EHISWaste.pdf to learn how to properly dispose of hazardous and non-hazardous materials in your workplace. ________________________________ Attention: Cal State Fullerton's area code is changing soon! Beginning Saturday, May 30, 2009, all Fullerton and Irvine campus phone and fax numbers will begin with 657-278 followed by the current extension number. _______________________________________________ Cellbiol mailing list Cellbiol@net.bio.net http://www.bio.net/biomail/listinfo/cellbiol This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From sucharitasen9 from yahoo.co.in Wed Apr 29 01:19:37 2009 From: sucharitasen9 from yahoo.co.in (sucharita sen) Date: Wed Apr 29 10:09:09 2009 Subject: [Cell-biology] Request Message-ID: <432141.14799.qm@web8705.mail.in.yahoo.com> ? Hello, I've to?detect apoptosis and necrosis?cell (assay) ?of a ?hybridoma cell line to detect toxic effect of ammonia and lactate on this cell. ? If you have any solution, would you please inform me that. ? with regards, sucharita PhD student Animal cell lab Dept of Biochemical Engg &Biotech IIT Delhi, India Now surf faster and smarter ! Check out the new Firefox 3 - Yahoo! Edition http://downloads.yahoo.com/in/firefox/?fr=om_email_firefox From csztalry from grecc.umaryland.edu Wed Apr 29 10:02:10 2009 From: csztalry from grecc.umaryland.edu (Carole Sztalryd-Woodle) Date: Wed Apr 29 10:09:42 2009 Subject: [Cell-biology] TDGA..where to get it?&In-Reply-To= Message-ID: <49F833AE.66C8.006C.0@grecc.umaryland.edu> I am also looking for it, did you get any answers? Carole Sztalryd Assistant Professor of Medicine Division of Endocrinology HH room 445A 650 West Redwwod Street Baltimore MD 21201 (office) 1-410-706-4047 (lab) 1-410-706-3505 or 3594 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Sztalryd-Woodle, Carole TEL;WORK:5-5417 ORG:;Med-EDN TEL;PREF;FAX:5-7913 EMAIL;WORK;PREF:csztalry@grecc.umaryland.edu N:Sztalryd-Woodle;Carole TITLE:Assistant Professor ADR;DOM;WORK;PARCEL;POSTAL:;HH LABEL;DOM;WORK;PARCEL;POSTAL;ENCODING=QUOTED-PRINTABLE:Sztalryd-Woodle, Carole=0A= HH END:VCARD