[Cell-biology] need your help
bhawana from chembiotek.com
(by bhawana from chembiotek.com)
Thu Aug 13 06:01:43 EST 2009
This is Dr Bhawana Gupta from India. I want to do transfection in floating
cells either in U937 or in HL60. I procured Fugene hd from Roche. Do you
have any experience of tranfecting floating cells and making stable cell
lines. I couldn't get any specific protocol regarding selection of these
tranfected floating cells and how to separate live cells from dead once,
because we can not spin such a lower no of cells during selection. If you
have related knowledge, please share with me. I am in acute need.
Thanks & Best Regards
Dr. Bhawana Gupta
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