[Cell-biology] need your help

bhawana from chembiotek.com via cellbiol%40net.bio.net (by bhawana from chembiotek.com)
Thu Aug 13 06:01:43 EST 2009


This is Dr Bhawana Gupta from India. I want to do transfection in floating 
cells either in U937 or in HL60. I procured Fugene hd from Roche. Do you 
have any experience of tranfecting floating cells and making stable cell 
lines. I couldn't get any specific protocol regarding selection of these 
tranfected floating cells and how to separate live cells from dead once, 
because we can not spin such a lower no of cells during selection. If you 
have related knowledge, please share with me. I am in acute need.

Thanks & Best Regards

Dr. Bhawana Gupta

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