[Cell-biology] Lung fibroblast methotrexate toxicity

Sonal Patel via cellbiol%40net.bio.net (by stylosweetu from gmail.com)
Sat Jun 9 09:24:38 EST 2012

I am trying to find out effect of methotrexate on lung fibroblast WT cells
and KO where gene of my interest is knocked out . After 3 weeks on staining
with crystal violet I get lot of background cells in KO alongwith colonies.
Can anyone suggest me how to remove the background cells.

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