Immunofluorescence

Cheryl A. Telmer ct2v+ at andrew.cmu.edu
Thu Nov 4 15:04:40 EST 1993


I am trying to stain WT Chlamy cells with a number of different
polyclonal antibodies.  As positive controls I have monoclonal
antibodies which stain beautifully but no specific signal is showing up
with the polyclonals.

There are two questions here.
1.  Is there a difference in antibodies to explain good monoclonal entry
and bad polyclonal entry?

2.  Does anyone have any tricks for working with polyclonals they are
willing to share?

Cheryl Telmer
Carnegie Mellon University
Pittsburgh PA




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