Cheryl A. Telmer
ct2v+ at andrew.cmu.edu
Thu Nov 4 15:04:40 EST 1993
I am trying to stain WT Chlamy cells with a number of different
polyclonal antibodies. As positive controls I have monoclonal
antibodies which stain beautifully but no specific signal is showing up
with the polyclonals.
There are two questions here.
1. Is there a difference in antibodies to explain good monoclonal entry
and bad polyclonal entry?
2. Does anyone have any tricks for working with polyclonals they are
willing to share?
Carnegie Mellon University
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