We are trying to screen a genomic lambda library containing DNA from an
alga with an unusually high GC content (about 70%). We have a homologous
probe (also with high GC) and are having trouble getting signals. Has
anybody had any experience similar to this? I know Chlamy also has high GC
and I was hoping someone might have some useful advice. We're thinking it
might have to do with incomplete denaturation, or reannealing of the probe,
as very high probe concentrations seem to help.
Thanks.
Terri Dunahay
dunahayt at tcplink.nrel.gov
