Heterologous Transformation Info Request

hwang at WUSTLB.WUSTL.EDU hwang at WUSTLB.WUSTL.EDU
Wed Sep 8 09:32:08 EST 1993


	A plant molecular biologist has indicated an interest in 
pursuing a systemmatic exploration of Chlamy transformation with 
heterologous, mostly higher plant, DNA.  The idea is to use the most 
up-to-date vectors and up-to-date transformation techniques and see if 
the problem can be solved.  Since he has considerable resources, this 
project could yield results of considerable benefit to all of us.
	Therefore, I am using the Internet to request from each of you 
the following:
	1. Published experiments describing efforts to express 
non-homologous genes, or use non-homologous promoters, in Chlamy.
	2. Unpublished experiments of the same ilk:  what you tried, 
what you found, including your mode of transformation, recipient 
strains, ets., anything that might be useful.
	3. Any observations you've made, or speculations you've 
entertained, as to why hereologous genes are not expressed.
	4. Published/unpublished experiments using antisense (homologous 
systems included).

Send replies to GOODENOUGH at WUSTLB.WUSTL.EDU

Thanks,
Ursula W. Goodenough




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