PCR with degenerate primers

Andy Wang andywang at VAXA.WEEG.UIOWA.EDU
Thu Sep 16 11:49:59 EST 1993


We have tried to do PCR of chlamydomonas DNA with degenerate primers. We have
followed the procedures suggested by a PCR book with some modification of
reannealing temperatures. We have always ended up with a large number of bands.
Has anyone tried and succeeded in using degenerate primers with Chlamy DNA?




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