Newsletter #38

Mike Adams adams at ECSUC.CTSTATEU.EDU
Tue Aug 2 09:38:43 EST 1994

July 1994

The Sixth International Chlamydomonas meetings at Lake Tahoe may be
remembered as the highest calorie meeting to date since, in addition to
outstanding food, there was a lot of free wine and beer. The almost 200
attendees from 20 countries sat through a packed program of talks,
including our first concurrent sessions on the last day. (A list of the
titles of all the talks and posters appears later in this Newsletter).

The next meeting will be May 27-June 1 1996 in Regensburg, Germany. The
organizers are Rudi Schmitt, Peter Hegemann and Stefan Fabry and Lib Harris
will act as the USA organizer. Additional information will be provided as
the planning gets under way

=46rom Lib Harris: The National Science Foundation has renewed support for
the Chlamydomonas Genetics Center for 5 years, beginning May 1994, but
because of budget cuts is requiring us to implement a cost-recovery
program.  Rather than charge for individual cultures, which we feel would
not be cost-effective, we are asking for an annual lump-sum payment from
every laboratory to whom we send cultures during the calendar year. For
1994 this charge will be $100 per laboratory for typical usage (on the
order of 1 to 5 mailings, totaling no more than about 50 cultures).  We
will consider requests for waiver of these charges on an individual basis.
To avoid the possibility of double or erroneous billing, please indicate
the name of a professor or laboratory head on each order.  We can address
packages to students or research staff, but we need to know whose lab to
bill at the end of the year. This fee will cover shipment by first class
mail.  We can also provide delivery via Federal Express, at your expense,
either by providing us with a FedEx account number to bill the recipient of
a package, or by reimbursing our costs for shipment. Typical charges for
overnight delivery within the US. are $8-11 depending on size of the
package. Delivery outside the US. is likely to run $20-40. Orders can be
placed by e-mail, phone, or fax.  No purchase order is necessary.

The new address for the Chlamydomonas Genetics Center is:

Chlamydomonas Genetics Center
c/o Dr. Elizabeth H. Harris
DCMB, Duke University
Box 91000
Durham NC 27708-1000

Our e-mail address will stay the same, chlamy at

phone 919-613-8164
fax 919-613-8177

=46or Federal Express and other deliveries requiring a street address, use
the following:

Chlamydomonas Genetics Center
c/o Dr. Elizabeth H. Harris
Levine Science Research Center
Research Drive
Duke University
Durham NC 27708-1000

In future, in order to reduce costs, the Newsletter will only be send to
people without email, unless you let me know that you really want to
continue getting a printed version. Each copy will be posted to the
chlamy.bionet and also placed at the Duke Gopher site. Once again, all
readers who can get onto the Internet and make use of these facilities are
urged to do so since it will allow them to get access to much more
information than is in the Newletter and do so much faster.

Several of the European labs have formed a consortium to work on gene
regulation in Chlamydomonas and may have openings for post-docs. The labs
include those of C. F. Beck (Freiburg), E. Fernandez (Cordoba), L.G.
=46ranz=E9n (Stockholm), U. K=FCck (Bochum), R. Matagne (Li=E8ge), J.D. Roch=
(Gen=E8ve), H. van den Ende (Amsterdam) and F.-A. Wollman (Paris) who is
acting as coordinator.
At the meetings Susan Dutcher raised the question of introducing a
consistent nomenclature for mutants in Chlamydomonas. At that time, and
since then on the bionet, there has been considerable discussion on the
topic. So far there does not seem to be a consensus but I will report on
any final decisions in a later issue.

=46rom: Don Weeks, UNL Center for Biotechnology, 101 Manter Hall, University
of Nebraska Lincoln, P.O. Box 880159, Lincoln NE  68588-0159 ( email:
dweeks at, fax  (402) 472-3139)
Our laboratory has recently constructed an indexed, cosmid library of
Chlamydomonas genomic DNA (Plant Molecular Biology 24, 663-672). We are now
in a position to make the library available to others in the Chlamydomonas
community.  The library, consisting of 11,280 members each in the
individual wells of 120 microtiter plates, may be useful for gene isolation
through genomic complementation or other procedures that benefit from
having a library in which individual members can be quickly and accurately
identified by number (i.e., by its position in a specific, single well of a
particular microtiter plate). The large number of clones in the library
provide a reasonable probability that any one particular Chlamydomonas gene
will be present. Thus, the calculated probability of finding at least one
copy of a large (10 kb) nuclear gene is approximately 92%. Additional
details concerning the construction of the library and its use for  gene
isolation by genomic complementation can be found in the publication cited
above. Technical delays in developing methods for the mass replication of
the library and in creating satisfactory means for shipping the library on
dry ice have delayed our plans for sharing the library at an earlier date.
Thus, some of you already have been aware of the existence of the library
and have requested copies.  We will honor those requests first if you will
let us know of your continued interest. Because grant funds were not
available to cover the notable expenses associated with replicating and
shipping the library, we are requesting that those wishing a copy of the
library pay a service fee of $1000 to partially cover the associated costs.
(If sufficient numbers of the library are requested to lower the per unit
cost of production, we may be in a position to offer the library to one or
two laboratories with no grant support or other means of paying for the
library.) Orders for the library will be honored on a first-come,
first-served basis. Questions and orders should be sent to Don at the
address/email/FAX shown above.
The talks and poster titles from the meetings are given below. They are
listed in the order of the program, so related talks should appear close to
each other.

D. Weeks, H. T. Zhang, P. Herman. An indexed cosmid library of
Chlamydomonas reinhardtii DNA for use in genomic complementation and gene

J. Nelson, P. Lefebvre. A dominant selectable marker for nuclear
transformation of Chlamydomonas reinhardtii. =E5

J. W. Jarvik, S. A. Adler, C. A. Telmer. CD-tagging: a new technology for
identifying and isolating genes, transcripts and proteins.

D. Straus, M. Rosenberg, M. Przybylska, E. Ananiev. A new method for
cloning genes known only by phenotype demonstrated in Volvox.

G. Gl=F6ckner, C. F. Beck. Cloning of a gene (LRG2) involved in the signal
transduction chain by which blue light controls sexual differentiation in

=46. Yildiz, J. P. Davies, A. R. Grossman. Acclimation of Chlamydomonas
reinhardtii to sulfur-limitation: the generation and characterization of
mutants constitutive for arylsulfatase expression.

R. Schmitt, I. Kobl, W. Mages, W. M=FCller, B. Schiedlmeier. Heterologous
expression of an arylsulfatase gene from Chlamydomonas in Volvox carten and
its use as a reporter gene

P. Liggit and E. Baker. Role of the 3' untranslated region in ubulin mRNA
stability and translation efficiency.

M. Vashishtha, J. L. Hall. Direct complementation of basal body/flagellar
mutations using YACs in Chlamydomonas reinhardtii.

D. Zhang, P. Lefebvre. Constitutive mutants for the expression of nitrate
reductase in Chlamydomonas reinhardtii.

R. A. White, J. K. Hoober. Biogenesis of thylakoid membranes in
Chlamydomonas reinhardtii y1. A kinetics study of initial greening.

Y. Bao, N. Deshpande, S. Hwang, D. L. Herrin. Light stimulates splicing of
the psbA premRNA in vivo

S. P. Mayfield, A. Danon, C. B. Yohn, A Cohen, R. Bruick, A. Lee. Light
activated translational regulation in C. reinhardtii chloroplasts.

U. Klein, L. Bogorad. Structure and activity of the promoter of the
Chlamydomonas chloroplast rbcL gene.

C. F. Beck, E. D. von Gromoff, J. Kropat. Light induction of hsp70 heat
shock genes of Chlamydomonas reinhardtii.

G. Small, B. Min, P. Lefebvre. Cloning a gene encoding a protein homologous
to an Arabidopsis blue-light photoreceptor.

H. Fukuzawa. Gene expression of carbonic anhydrase in Chlamydomonas
reinhardtii regulated by environmental C02 and light

A. R. Memon, S. Hwang, N. Deshpande. G. A. Thompson, D. L. Herrin.
Preferential expression and accumulation of ADP-ribosylation factor (ARF)
during growth and cytokinesis in Chlamydomonas reinhardtii.
L. L. Ehler, S. K. Dutcher. Characterization of Chlamydomonas reinhardtii
mutant strains defective in cytokinesis.

T. Saito, T. Shimada, K. Ashida, Y. Matsuda. The signals for daughter cell
liberation in the Chlamydomonas cell cycle.

H. van den Ende. Gametogenesis in Chlamydomonas monoica.

B. E. Taillon, D. L. Kirk. Towards a molecular dissection of early
embryonic development in Volvox carteri.

V. Kurvari, F. Qian, H. Gibbons, W. Snell. Identification of mRNA
transcripts induced during fertilization in Chlamydomonas.

P. J. Ferris, U. W. Goodenough. Characterizing the Chlamydomonas mating
type locus.

A. M. Campbell, H. J. Rayala, C. Stromquist, U. W. Goodenough. Sex-limited
mutation iso-1 defines gene outside the mating-type locus that is
responsible for minus dominance and sex-specific expression of
Chlamydomonas gametic genes

E. V. Armbrust, A. Ibrahim, U. W. Goodenough. A coupling between cell size
control and chloroplast genome inheritance in Chlamydomonas reinhardtii.

B. D. Kohorn, T. Smith. Mutations in a signal sequence for the thylalkoid
membrane identify multiple protein transport pathways and nuclear

L.-G. Franz=E9n, G. Nurani, M. Hugosson, E. Glaser. Biogenesis of
nuclear-encoded chloroplastic and mitochondrial proteins in Chlamydomonas

R. W. Lee, E. M. Denovan-Wright, D. F. Spencer. Fragmented mitochondrial
ribosomal RNAs in Chlamydomonas.

B. L. Randolph-Anderson, J. E. Boynton, N. W. Gillham. Suppressors of
streptomycin dependence in Chlamydomonas reinhardtii.

S. Hong, R. J. Spreitzer. Nuclear mutation reduces transcription of the
rbcL chloroplast gene that encodes the large subunit of
ribulosebisphosphate carboxylase/oxygenase

M. Goldschmidt-Clermont, F. D=FCrrenberger, V. Loroch, J.-D. Rochaix. Nuclea=
factors required for chloroplast gene expression: psaA trans-splicing

K. L. Kindle, H. Suzuki, D. Stern. Suppression of an unstable atpB mRNA
phenotype can occur by distinct mechanisms.

K. Jones, E. Beasley, L. Mets. 24DD, a nuclear mutation affecting the
Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene (rbcL)
mRNA stability in Chlamydomonas reinhardtii.

H. Lee, A. N. Webber, S. E. Bingham. Role of the stem-loop structure and
translation termination in control of degradation of mRNA from the
chloroplast psaB gene.

0. A. Sodeinde, D. Stern, K. L. Kindle. Isolation of nuclear genes involved
in post-transcriptional regulation of the chloroplasts petD gene.

J. P. Woessner, S. Waffenschmidt, U. W. Goodenough. Studies on
Chlamydomonas reinhardtiicell wall assembly using regenerating vegetative
cells or developing zygotes.

Y. Matsuda, T. Kinoshita, T. Saito. Synthesis, storage and activation of
gamete lytic enzyme in Chlamydomonas reinhardtii.

S. Waffenschmidt. O-glycosylation in Chlamydomonas reinhardtii.

M. Viskaduraki, C. Forest. Vegetative and gamete-specific proteins in
Chlamydomonas plasma membrane.

C. Forest. Fusion inhibition by surfactants suggests a virus-like fusion
peptide may be involved in Chlamydomonas reinhardtii fertilization

N. Libessart, C. D'Hulst, M.-L. Maddelein, B. Delrue, N. van den
Koornhuyse, A. Decq, S. G. Ball. Semi-dominant high amylose mutants of
Chlamydomonas reinhardtii display reduced phosphorylase, branching enzyme
and modified starch synthase activities

J. P. Davies, F. Yildiz, A. R. Grossman. Generation, characterization. and
complementation of mutants with aberrant responses to sulfur-deprivation

O. Vallon, L. Bult=E9, F.-A. Wollman. The periplasmic amino-acid oxidase of
Chlamydomonas reinhardtir molecular cloning and regulation of its

E. Fern=E1ndez, A. Quesada, A. Galv=E1n. Function of nar genes clustered wit=
the nitrate reductase gene from Chlamydomonas.

R. Schnell, P. Lefebvre. Structure and function of NIT2, a pathway-specific
regulatory gene in Chlamydomonas.

S.-C. Wang, R. Schnell, M. Prigge, P. Lefebvre. Characterization of NIT8, a
gene required for nitrate assimilation in Chlamydomonas.

=46. Merch=E1n, R. Prieto, A. Galv=E1n, E. Fernandez. What is a DNA like=
 this one
doing in an organism like Chlamydomonas?

C. Wieland, L. van de Burght, L. R. Keller. Excision of Chlamydomonas
reinhardtii flagella using an optical trap system

M. Adams, E. Savino. Induction and identification of hsp70 following heat
shock and deflagellation

W. L. Dentler, J. Lappin. Cell-cycle regulated flagellar shortening in
Chlamydomonas reinhardtii.

H. J. Hoops, D. Nydam, K. Feathers, M. Brighton. Polarity of the flagellar
apparatus is correlated with flagellar motion and swimming behavior in
Volvox carteri.

L.-W. Tam, P. Lefebvre. Molecular characterization of two Chlamydomonas
genes involved in flagellar motility.

S. K. Dutcher, S. Schuyler, S. J. King, E. C. Trabuco. Characterization of
two new UNI loci in Chlamydomonas reinhardtii.

S. K. Dutcher, L. L. Ehler, A. M. Preble, A. L. Palombella. The phenotypes
of the bld2 mutation.

C. M. Asleson, P. Lefebvre. The identification of new loci involved in
flagellar length control in Chlamydomonas reinhardtii.

D. Diener, J. L. Rosenbaum. In vivo analysis of sequences involved in
targeting and binding of radial spoke protein 3 to the flagellum.

D. R. Howard, G. Habermacher, W. S. Sale. Flagellar dynein activity is
inhibited by an endogenous axonemal kinase in spoke-deficient axonemes.

M. Bernstein, P. L. Beech, J. L. Rosenbaum. A new kinesinlike protein
(Klp1) localized to a single microtubule of the Chlamydomonas flagellum.

E. F. Smith, P. Lefebvre. A molecular approach to understanding central
apparatus function in Chlamydomonas flagella.

C. Beck, R. Uhl. On the localization of voltage-sensitive calcium channels
in the flagella of Chlamydomonas reinhardtii.

G. J. Pazour, G. B. Witman. Genetic analysis of the phototaxis signal
transduction pathway in Chlamydomonas.

S. J. King, S. K. Dutcher. Characterization of cis/trans flagellar
asymmetry through a mutational analysis.

M. R. Lamb, J. Ihle, C. L. Dieckmann. A genetic and ultrastructural
analysis of phototactic mutants of Chlamydomonas reinhardtii.

P. Kr=F6ger, Y. Zhang, P. Hegemann. Localization of rhodopsin by

K. Yoshimura. The orientation of photoreceptor as probed by cells' response
to polarized light.

E. Ermilova, E. M. Chekunova, K. Krupnov, B. Gromov. Chemotactic mutants of
Chlamydomonas reinhardtii defective in responses to sugars.

R. Starr, L. Jaenicke. Chemo-attraction of male gametes by female gametes
in Chlamydomonas.

L. M. Quarmby, H. C. Hartzell. Two different calcium signalling pathways
induce deflagellation in Chlamydomonas reinhardtii.

J. H. Evans, J. L. Cheshire, L. R. Keller. CA2+ signaling in the
Chlamydomonas reinhardtii flagellar regeneration system: cellular and
molecular responses.

L. R. Keller, J. L. Cheshire. Protein kinase activity in Chlamydomonas
reinhardtii flagellar regeneration.

R. A. Bloodgood, N. L. Salomonsky. Flagellar membrane associated
phosphoproteins are involved in transmembrane signal transduction.

T. Munnik, J. Brederoo, S. A. Arisz, R. F. Irvine, A. Musgrave. Signs of
phospholipase C. phospholipase D and phosphoinositide 3-kinase signalling
in Chlamydomonas.

M. Siderius, J. van Himbergen, H. Kuin, A. Musgrave. Calcium-dependent
kinases and calcium stores in C. eugametos gametes.

M. A. Haring, M. Siderius, A. Musgrave. Plant tyrosine phosphatase
signalling: oxidative stress and cell-cycle regulated expression of the
Chlamydomonas eugametos VH-PTP13 gene

W. Snell, Y. Luo, Y. Zhang. Flagellar adhesion-dependent regulation of
Chlamydomonas adenylyl cyclase in vitro: a possible role for protein
kinases in sexual signalling

R. J. Spreitzer, S. Hong. Long and short-range interactions affect the
C02/02 specificity of chloroplast ribulosebisphosphate
carboxylase/oxygenase (Rubisco)

S. Ross, M. Zhang, B. Selman. Site-directed mutagenesis of the y-subunit of
the C. reinhardtii coupling factor (CF1)

K. Hill, S. Merchant. Coordinate expression of pre-apocyt c6 and
coproporphyrinogen oxidase in copper-deficient Chlamydomonas reinhardtii

J. M. Quinn, S. Merchant. Identification of copper-responsive elements
(CREs) in the cytochrome c6 promoter of Chlamydomonas reinhardtii.

L. Cui, S. E. Bingham, S. Gulbranson, A. N. Webber, S. M. Rodday, J.
Biggins. Mutational analysis of the photosystem I reaction center.

Y. Takahashi, H. Matsumoto, M. Goldschmidt-Clermont, J.-D. Rochaix.
Construction and characterization of mutants with disrupted chloroplast
psbK gene of C. reinhardtii.

N. J. Gumpel, S. Purton. Photosynthetic genes of Chlamydomonas reinhardtii
identified by insertional mutagenesis using the ARG7 marker.

A. Tr=E9moli=E9res, G. Dubertret. In vivo manipulation of lipid composition =
Chlamydomonas: a tool to study the role of lipids in the biogenesis of the
photosynthetic membrane.

G. L. Matters, S. 1. Beale. Complementation cloning, sequence and
light-regulated expression of two heme and chlorophyll biosynthetic genes
from Chlamydomonas reinhardtii.

J. Li, M. P. Timko. Characterization of NADPH: protochlorophyllide
reductase gene expression in wild-type and mutant Chlamydomonas

E. M. Chekunova, N. V. Shalygo, E. B. Yaronskaya, N. G. Averina, A. S.
Chunayev. A novel chloroplast gene mutation affecting chlorophyll
biosynthesis in Chlamydomonas reinhardtii.

A. R. Zvintchouk, E. F. Salimova, Z. Bolathan, R. Kr=F6ning, A. Boschetti, A=
S. Chunayev. Norflurazon-resistant mutants in Chlamydomonas reinhardtii.

D. W. Bollivar, S. 1. Beale. Isocyclic ring-forming reaction of chlorophyll
biosynthesis in intact Chlamydomonas reinhardtii chloroplasts isolated
using the BioNeb cell disruptor.

C. Silflow, P. Kathir, P. Lefebvre. Molecular mapping of the Chlamydomonas
reinhardtii nuclear genome

=46. Schuring, P. J. Ferris, J. Urbanus, M. A. Haring. Use of random
amplified polymorphic DNA markers (RAPD's) for locating genes in

A. Day, T. Hails, 0. Huttner, D. Pike. Analysis of Chlamydomonas
reinhardtii chromosomes

J. M. Erickson, J. Kato, J. Lau, J. Rahimian, W. Harenburg. Homologous
recombination within the inverted repeat of the chloroplast genome:
characterization of deletion/insertion junction sites in psbA transformants
and mutants of Chlamydomonas reinhardtii.

J. Berman, S. Enomoto, L. Konkel, E. Chamberlain, P. Zierath. Phenotypic
analysis of Chlamydomonas gene by functional complementation in

S. Takada, R. Kamiya. Functional reconstitution of outer dynein arms from
a-b and g subunits.

S. M. King, G. B. Witman. Multiple sites of phosphorylation within the a
heavy chain of outer arm dynein.

S. Myster, J. Knott, S. Farlow, M. E. Porter. Characterization of the
dynein gene family in Chlamydomonas reinhardtii.

D. Mitchell. Characterization of new alleles at flagellar outer row dynein
assembly (oda) loci created by insertional inactivation mutagenesis.

C. Wilkerson, S. M. King, G. B. Witman. Molecular analysis of the g heavy
chain of Chlamydomonas flagellar outer-arm dynein.

C. Perrone, L. Gardner, E. T. O'Toole, T. Giddings, M. E. Porter.
Components of the "dynein regulatory complex" reside at the junction
between the radial spokes and the inner dynein arms.

G. Piperno, K. Mead, M. LeDizet. Mutations in the "dynein regulatory
complex" alter the ATP-insensitive binging sites of inner arm dyneins in
Chlamydomonas axonemes

M. LeDizet, G. Piperno. Characterization of the gene for P28, a light chain
of inner dynein arms 12 and 13 in Chlamydomonas.

R. L. Shirley, S. J. King, E. T. O'Toole, S. K. Dutcher. Inner dynein arm
mutations: isolation of new bop2 alleles.

U. Johanningmeier, S. Schrader, J. Schmidt, G. Huch, T. Tornow, U.
Altenfeld. Key mutants for the analysis of the D1-subunit of photosystem II

K. J. van Wijk, S. Styring. Reactivation of photosystem II after
photoinhibition in Chlamydomonas reinhardtii.

E. Bracht, A. Trebst. Evidence for a cleavage enzyme in the rapid turnover
of the D1 protein of photosystem 11, regulated by a redox controlled

R. Hutchison, J. Xiong, R. T. Sayre, Govindjee. Site-directed mutagenesis
of a bicarbonate binding site arginine 269 of the D1 protein in
Chlamydomonas reinhardtii.

A. Lardans, B. Forster, N. W. Gillham, J. E. Boynton. Effects of specific
amino acid replacements at position 251 of the D1 photosystem 11 protein in
Chlamydomonas reinhardtii.

M. Adam, R. Loppes. Isolation of phosphatase-negative mutants by insertion
of transforming DNA in Chlamydomonas reinhardtii.

G. Choi, M. Przybylska, D. Lifschutz, D. Straus. Identity of Volvox
cell-type-specific genes.
S. Fabry, A. Lindauer, T. Cornelius, H. Huber, R. Klose, M. Bruderlein, R.
Schmitt. Examination of histone H1 genes and their gene products in Volvox
carten and Chlamydomonas reinhardtii.

S. Fabry, R. Schmitt, M. M. Kirk, D. L. Kirk. Nucleotide sequences of
introns in genes that encode small G proteins in C. reinhardtii and some of
its relatives: a tool for high-resolution phylogenetic analysis?

E. Fernandez, A. Galvan, R. Prieto. Identification of putative regulatory
genes for the nitrate assimilation pathway.

P. J. Ferris, U. W. Goodenough. Cloning genes in Chlamydomonas by
chromosome walking.

E. H. Harris, G. L. Hughes. A public database for information on Chlamydomon=

J. McHugh, J. Spanier. The use of transformation/insertional mutagenesis to
isolate cadmium sensitive mutants in Chlamydomonas reinhardtii.

S. Miller, M. M. Kirk, D. L. Kirk. Transposon tagging strategies for Volvox

S. Nikaido, C. Locke, D. Weeks. Automated sampling and RNA isolation at
room temperature for measurements of circadian rhythms in gene expression
in Chlamydomonas reinhardtii.

A. L. Palombella, L. Lenz, S. K. Dutcher. The identification of an
Escherichia coli host strain that maintains a Chlamydomonas DNA sequence
that is unstable in many other strains

A. Sizova, V. N. Danilenko, N. N. Aleksandrova, K. E. Akopianz, T. V.
Lapina, A. V. Kozlov. A new nuclear marker of resistance to aminoglycoside
antibiotics for Chlamydomonas reinhardtii

A. Sizova, T. V. Lapina, A. V. Kozlov. Analysis of Chlamydomonas
reinhardtii nuclear DNA fragment from a plasmid that maintains autonomously
in C. reinhardtii cells.

D. Stevens, S. Purton. Expression of foreign genes in Chlamydomonas

E. C. Trabuco, S. K. Dutcher. A search for gene disruption events at g-tubul=

Q. Chen, C. Silflow. Isolation and characterization of glutamine synthetase
genes in Chlamydomonas reinhardtii.

C. Dunn, K. VanWinkle-Swift. Chlamydomonas monoica strains showing altered
nitrate assimilation

E. Fernandez, N. L'vov, K. Kalakoutskii. Purification and characterization
of a protein related with molybdate metabolism in Chlamydomonas.

N. van den Koornhuyse, S. G. Ball, J. Preiss. Genetic control of
Chlamydomonas reinhardtii
        ADP-glucose pyrophosphorylase

J. H. Waterborg, D. L. Tatar, C. M. Borza. Protein sequence analysis of
site-specific acetylation and methylation in Chlamydomonas histone H3
reveals a novel histone H3 protein

D. A. Berthold, 1. Baroli, J. Fernandez-Velasco, R. Malkin. Removal of the
"extra" threonine between the b hemes destabilizes the cytochrome bf
complex of Chlamydomonas reinhardtii.

N. R. Bertos, S. P. Gibbs. Localization of photosystem I to both the
appressed and non-appressed thylakoid membranes of Chlamydomonas
reinhardtii chloroplasts.

V. Borodin, G. Samuelsson, P. Gardestrom. The effect of light quality on
adaptation of Chlamydomonas reinhardtii to low carbon conditions.

Y. Eisenberg-Domovich, I. Ohad, K. Kloppstech. Membrane association of
HSP22 of Chlamydomonas reinhardtii during heat-shock and its subsequent
dissociation during recovery.

R. Hutchison, H. Wincencjusz, R. T. Sayre. Isolation of a photosystem 11
reaction center particle from Chlamydomonas reinhardtii.

S. Jacobshagen, K. L. Kindle, C. H. Johnson. Rhythms of cab ll, b-tubulin
and cytochrome c mRNA abundance in Chlamydomonas - are they truly
circadian, independent of the cell division cycle, and transcriptionally

M. Stein, E. Jeannette, J.-P. Jacquot. Organization of thioredoxin genes in
Chlamydomonas reinhardtii.

T. Knott, A. Creighton, C. Robinson. The biogenesis of proteins destined
for the thylakoid lumen of Chlamydomonas reinhardtii.

L. Mets, X. S. Xie, R. C. Dunn, M. Du, G. R. Fleming. Ultrafast time
resolution measurement of energy transfer dynamics and near-field high
resolution fluorescence imaging of LHC complexes in the photosynthetic
membranes of Chlamydomonas reinhardtii.

J. Minagawa, A. Crofts. Site-directed mutagenesis of the photosystem 11
reaction center-construction of PCR-spliced psbA gene in a plasmid
conferring spectinomycin resistance and the application to mutagenesis at
the donor side.

T. Mor, 1. Ohad, H. Pakrasi. psbE and psbF form separated transcription
units in Chlamydomonas reinhardtii.

K. K. Niyogi, O. Bj=F6rkman, A. R. Grossman. Isolation and characterization
of mutations affecting photoprotection in Chlamydomonas reinhardtii.

H. Connor, J. Nugent, S. Purton. The function of the 9 kDa phosphoprotein
of photosystem II: a reverse genetics study.

L. Ralley, N. J. Gumpel, S. Purton. Characterization of nuclear mutants of
C. reinhardtii defective in photosynthesis

R. A. Roffey, D. M. Kramer, Govindjee, K. J. van Wijk, S. Styring, R. T.
Sayre. Modification of photosystem II electron transfer by lumenal side
histidine mutations in the D1 protein in Chlamydomonas reinhardtii.

T. Happe, D. Naber, A. Trebst. Characterization of a hydrogenase from
Chlamydomonas reinhardtii.

J. Zhou, R. Malkin. Site-directed mutagenesis of the petA gene encoding
cytochrome f in Chlamydomonas reinhardtii.

G. Habermacher, D. R. Howard, W. S. Sale. Calcium control of dynein driven
microtubule sliding in isolated flagellar axonemes.
T. Kato, O. Kagami, T. Yagi, R. Kamiya. Isolation of two flagellar mutants,
ida5 and ida6, that lack subsets of inner-arm dynein heavy chains.

R. W. Linck. What sea urchin tektins can tell us about Chlamydomonas and
vice versa.

B. F. Mitchell, D. McDermott, T. Halstead. The isolation and
characterization of motile secondary mutants in Chlamydomonas reinhardtii
carrying the pf1 mutation.

M. A. Sullivan, M. E. Porter, R. W. Linck. Molecular cloning of tektin
proteins in Chlamydomonas reinhardtii.

W.-y. Wang. Genetic analysis of phototactic behaviors of Chlamydomonas

C. D. Amundsen, P. Lefebvre. Chlamydomonas reinhardtii flagellaless mutants

W. L. Dentler, J. Lappin. Cell-cycle regulated flagellar shortening in
Chlamydomonas reinhardtii.

C. Horst, G. J. Pazour, G. B. Witman. A new mutant strain of Chlamydomonas
which is defective in microtubule organization

L. Huirong. New techniques--a method to observe the zoospores and motile
cell (Chlamydomonas) on the slot agar plate.

A. Kernan, C. Silflow. Isolation and characterization of Chlamydomonas
reinhardtii mutants defective in flagellar regeneration following

W.-C. Wu, L.-W. Tam, C. Silflow. Isolation and characterization of an
abnormal flagellar-number mutant generated by insertional mutagenesis

L. Bulte, O. Vallon, F.-A. Wollman. Chlamydomonas reinhardtii response to
amino-acids as a sole source of nitrogen.

R. Donald, W. Cruz, K. VanWinkle-Swift. Analysis of theChlamydomonas
monoica zygospore wall

J. Lew, L. Konkel, M. Kable, J. Berman. Domain characterization and
substrate specificity of Chlamydomonas reinhardtii telomere ss-G-strand
binding protein.

0. Vallon, F.-A. Wollman. Mutations affecting o-glycosylation and causing
palmelloid colonies prevent sexual agglutination of mt+ cells only

N. Wilson, G. Huang, W. Snell. Analysis of the fertilization tubule in
Chlamydomonas by cell fractionation and insertional mutagenesis.

J. M. Escoubas, J. LaRoche, P. G. Falkowski. Light-regulated transcription
of Dunaliella tertiolecta cab genes.

C. Forest. Detergent stimulation of flagellar agglutination and zygote

C. Nonneng=E4sser, H. Harz, E. Holland, P. Hegemann. The ionic nature of
rhodopsin mediated photocurrents.

H. Harz, R. Uhl, P. Hegemann. Direct correlation between rhodopsin mediated
photocurrents and flagellar beeting changes

H. Salawu, M. Viskaduraki, C. Forest. Biotinylation and detection of
Chlamydomonas reinhardtii plasma membrane proteins

K. Schaller, R. Uhl. On the mechanism of phototactic orientation in
Chlamydomonas reinhardtii.

R. Drager, G. Schuster, D. Stern. Endonucleolytic processing of mRNA in
Chlamydomonas reinhardtii chloroplasts.

B. Dreyfuss, J. M. Erickson. An inverted repeat in intron 1 from the
Chlamydomonas reinhardtii psbA gene is not necessary for mRNA splicing in

J. Girard-Bascou, Y. Choquet, N. J. Gumpel, D. Culler, A. Lardans, R.
Kuras, S. Merchant, S. Purton, F.-A. Wollman. Nuclear control of the
expression and assembly of the major chloroplast subunits of cyt b6/f
complex in Chlamydomonas reinhardtii.

D. Gonz=E1lez-Halphen, A. Antarami=E1n, R. Coria. Comparative analysis of
Chlamydomonas reinhardtii and Polytomella spp. mitochondrial COXI gene.

N. Deshpande, M. Hollingsworth, D. L. Herrin. The atpF group II intron from
spinach chloroplasts is not spliced in transgenic Chlamydomonas

L. Mets, C. Roitgrund. Homologous intermolecular recombination may play a
key role in the origin of deletion/duplications that extend the length of
the large inverted repeat in chloroplast DNA.

G. Nurani, L.-G. Franz=E9n. Purification of mitochondrial ATP synthase from
Chlamydomonas reinhardtii and isolation of a cDNA clone of the alpha

C. Remacle, M.-P. Dorthu, M. Colin, M.-R. Michel-Wolwertz, R. F. Matagne.
Genetic mapping of mitochondrial markers by recombination analysis in
Chlamydomonas reinhardtii.

V. Rukmini. Coordination of chloroplast and mitochrondrial genome
copy-numbers vis-a-vis photosynthesis and respiration in Chlamydomonas

H. Uchida, S. Kawano, N. Sato, T. Kuroiwa. Isolation and characterization
of novel genes which are expressed during the very early stage of zygote
formation in Chlamydomonas reinhardtii.

Z. Xie, S. Merchant. Molecular analysis of the candidate of the cytochrome
c lyase gene in Chlamydomonas reinhardtii

Mike Adams
Biology Dept, ECSU
Willimantic, CT 06226
(203) 465-5305

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