Labelling with amino acids

Christopher Yohn cyohn at scripps.edu
Mon Jan 3 20:00:26 EST 1994


Does anyone have experience labelling Chlamydomonas in vivo with
radiolabelled amino acids (i.e. 3H or 14C)?  Will the amino acids be taken
up from the culture media in sufficient quantity to do a pulse labelling of
protein synthesis?  Is it necessary to toluene-permeabilize the cells to
accomplish this?  Any protocols, tips, hints, etc. will be greatly
appreciated.  Thanks in advance.

Christopher B. Yohn
Dept. of Cell Biology - MB8
The Scripps Research Institute
La Jolla, CA  92037
Ph:  (619) 554-4335
FAX: (619) 554-6188
cyohn at scripps.edu






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