chlamy growth conditions?

unknown at dl.ac.uk unknown at dl.ac.uk
Fri Jan 7 08:59:29 EST 1994


>Hello netters:
>What are the preferred growth conditions for "wild type" chlamy? (media, temp)
>All suggestions are welcome.
>Antonio Reinero
>Plant Biotech- American Cyanamid

Temperatures in the 20-25 degree range usually are best.  There are three
media that are widely used:

TAP [Tris-acetate-phosphate], from Gorman and Levine, Proc. Natl. Acad.
Sci. USA 54, 1665

Sueoka high salt medium, from Sueoka, Proc. Natl. Acad. Sci. USA 46, 83

Sager and Granick medium, from their 1953 paper in Ann. N.Y. Acad. Sci. 56,
831


Recipe sheets can be obtained from the Chlamydomonas Genetics Center at
Duke (chlamy at acpub.duke.edu), and are also in _The Chlamydomonas
Sourcebook_.

One word of caution: the most widely used wild type strain, 137C, lacks
nitrate reductase activity and must be supplied with a reduced nitrogen
source, normally ammonium.  The Sueoka and TAP media have all their
nitrogen as NH4.  However, Sager and Granick medium as originally
formulated contains NH4NO3, and supplementation with extra NH4 is advisable
when growing the 137C strain.

Elizabeth Harris
chlamy at acpub.duke.edu





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