Biolistic transformation using helium gan

surzycki at SUNFLOWER.BIO.INDIANA.EDU surzycki at SUNFLOWER.BIO.INDIANA.EDU
Wed Jul 6 11:42:06 EST 1994


Does anybody used new Bio-Rad helium gun to transform Chlamydomonas
chloroplast. If yes, what are the optimal conditions for transformation
using PDS-1000/He gun. What is optimal He pressure, target distance, type
of rupture disk etc. Are gold particles better than tungsten particles for
chloroplast transformations? 

Stefan Surzycki





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