regarding seeing flagella - either close down the condenser aperture or
make a partial condenser back focal plane stop that causes some shear.
Put it over the bottom of the Abbe condenser just in back of the
condenser aperture and you're in like Flynn.
That produces oblique illumination and our forefathers in the previous
century used this method (related to Hoffmann mod. contrast and Single
Sideband Enhanced Contrast - Ellis's method, but much cruder) to see all
sorts of wonderful cellular details. Ever wonder how they saw all that
stuff and yet didn't have nice Zeiss micrscopes with DIC or phase?
That's how. It cuts down on light, but usually the scopes deliver much
more light than you need anyhow.
On 20 Jun 1994 chlamy at acpub.duke.edu wrote:
> Bill Snell is interested in experiments that can be done with Chlamydomonas
> in a high school laboratory (low budget, minimal equipment, no
> health/safety hazards). I've offered to compile as many ideas as people
> can come up with, and put them on the gopher server. Any suggestions? I
> enclose part of Bill's letter to me:
>> >I have a high school biology teacher/football coach working in my lab this
> >summer. We've talked about possible ways to incorporate Chlamy into his
> >classroom. Do you have any suggestions
> >about exercises for high school students. Also, do you know of a way to stain
> >cells so that their flagella can be seen in a bright field microscope. Our teacher tells me that he thinks most high schools will not have a phase
> >contrast microscope. We might be able to see them with iodine, but because
> >iodine can sublimate, I'm not sure it would be a good idea to have it sitting
> >around a high school lab. Any suggestions or ideas from you will be welcomed.
>> Elizabeth Harris
>chlamy at acpub.duke.edu>>>