pf dikaryon rescue, student lab exercise

chlamy at ACPUB.DUKE.EDU chlamy at ACPUB.DUKE.EDU
Fri Jun 24 11:22:31 EST 1994


>I teach an undergraduate cell biology laboratory and am trying to think
>up some new "investigative labs" to add to the mix.  While attending the
>Chlamydomonas meetings, some of the papers reminded me of the concept of
>dikaryon rescue of the motile phenotype.  It struck me that this could
>be the basis of an interesting laboratory.
>My tentative plan would be to have two different pf mutants (of different
>mating type) capable of complementing each other.  After mating one should
>see restoration of motility of each flagellar pair.  With the proper leading 
>questions, one should be able to get the students to figure out the
>general basis for the restoration of motility.
>It would be most useful to have 2 easily scoreable but different
>mutant phenotypes.  If necessary we could cross one or more mutants with 
>wild type, but I think that undergraduates would be able to score absence
>vs. presence of motility in the gametes and zygotes more easily than they
>could tell if 2 or 4 flagella in a zygote were motile.  It would also be nice
>to have two strains that will mate but not complement (plus and minus 
>strains of the same pf mutant?).
>The lab, would lend itself to asking questions dealing with the requirement
>for new message or protein synthesis etc. using inhibitors.
My questions follow:
>1)   Do you know of any elementary laboratory exercises that follow this 
>general outline?
>2)   If not, what would be the best strains to use?
>3)   Is there anything tricky about using these strains of getting dikaryon
>rescue.  (I have experience in growing Chlamy, including pf mutants, and in
>mating wild-type cells.)
>     Thank you very much for your help (and your patience).  If I do make this 
>into a good lab. I would, of course, be willing to share it with anyone who
>Harold Hoops
>Associate Professor
>1 College circle
>Geneseo, NY 14454
>phone: (716) 245-5378
>e-mail: Hoops at uno.cc.geneseo.edu
Elizabeth Harris
chlamy at acpub.duke.edu

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