Nucleic acid extraction and library

"Cherry Steffen ", BIO steffen at CHUMA.CAS.USF.EDU
Fri Nov 11 10:43:08 EST 1994


Is it possible to use a guanidinium isothiocyanate method to extract 
nucleic acids from chlyamdomonas?


Also, I am a beginning graduate student and I am looking for a cDNA 
library to use to screen to help us isolate the lamin gene(s) in 
chlamydomonas.  Is there one avaliable?  How can I get my hands on one 
without major expense ?  
I am new at this aspect of science and would appreciate any help and 
suggestions that I can get.  Thanks in advance.

Cherry O. Steffen
University of South Florida




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