I have received several requests for recent information on
transformation of Chlamydomonas with silicon carbide whiskers. I have
not worked on this project since I published an article in
Biotechniques last year. Is anyone out there using silicon carbide
whiskers to transform Chlamydomonas? What is the source of your
fibers? I sent a notice that Alfa Aesar was selling SiC commercially,
and I did one experiment with their whiskers (they worked but gave a
low transformation frequency), but haven't gone back and tested them
rigorously. I would love to hear if any one else is using these or
any other "brand" of SiC with good results (or any results). Please
drop me a note (you can send it to me personally so the net won't get
cluttered) and I will collate and send out any info I receive.
Thanks for your help.
National Renewable Energy Lab
e-mail: dunahayt at tcplink.nrel.gov