mRNA preparation without intermediate total RNA preparation

Dr. F. Walter Frederek.Walter at uni-koeln.de
Wed Dec 6 09:13:27 EST 1995


Dear Netters,
I would like to isolate mRNA from Chlamydomonas reinhardtii without inter-
mediate total RNA preparation. For this purpose I thought to use the Oligotex
direct mRNA Kit provided by Qiagen. Does anyone has experiences with this kit
or with another one ? I only know that people working with animal tissues have
reached good results with these kinds of kits. So if anyone has experiences
with the preparation of mRNA in Chlamydomonas by this way I would be happy 
to hear about it.

With many thanks

Frederek Walter




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