Hai I am a graduate student developing some expression system in
Chlamydomonas. I am intending to attach an epitope for the protein
so that I could follow the cell cycle behavior of this protein. One
strong contender is Hemagglutinin. This is a short peptide to which
antibody has been developed and also shown to work in the Chlamydomonas
system.
The following is the sequence of the peptide (HA1 75-110)
Y P Y D V P D Y A nonamer
An antibody to this peptide was developed by Jeffrey Field and has
been published in Mol & Cell Biol of 1988 vol8 No5 p 2159-2165.
This epitope was chosen the above author based on the work of
Green et al Cell 1982 28 : 477-487
Niman et al PNAS 1983 80 : 4949-4953
Wilson et al Cell 1984 37 : 767-778
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The reason I am posting is if anybody on the net knew of a commercial
source for antibodies against hemagglutinin and it's epitopes. I would
be greatful if I could find a commercial source. Thank you very much.
errabolu at mayo.edu
