Isolating a nuclear protein

"Cherry Steffen ", BIO steffen at CHUMA.CAS.USF.EDU
Wed Jan 18 11:14:29 EST 1995


I am attempting to isolate a nuclear protein from Chlamydomonas 
reinhardtii.  The nuclear prep in the Chlamydomonas Sourcebook works 
great (except that my pellet is still green).  However, when I try to 
isolate the protein, I end up with a pellet so sticky that I cannot 
solubilize it.  I am incubating it in 0.02N NaOH and then in 30% TCA.  
Before the TCA the pellet is impossible to get into solution.  Does 
anyone have any ideas as to how I could isolate a nuclear protein from 
chlamy?  I would appreciate any help!  Thank you in advance.

Cherry O. Steffen
University of South Florida





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