counting chlamy

Elizabeth Harris chlamy at acpub.duke.edu
Thu May 4 08:06:30 EST 1995


> Hi!  Could anybody advise me on the kind of hemocytometer that  is usually
> used to count Chlamy?

We use an AO Spencer #1492 hemocytometer, with an ordinary compound
microscope and 10x objective. Immobilize the cells with a drop (per ml) of
iodine/alcohol solution.  If you have access to a copy of _The
Chlamydomonas Sourcebook_, take a look at the diagram on page 34.  This
shows what the grid looks like and which squares you should count.

-- 
Elizabeth Harris
chlamy at acpub.duke.edu




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