Now I am using auxtrophic strain, detecting the newly synthesized protein
induced by oxidative stress by labeling arginine with 14C. But in this way,
cells are given arginine stress, and it is the oxidative stress I would
like to see .
If there is a method that i can use wild type strains instead, I think it
will work out better. So if anybody can give me some advice, I would
appreciate it.
And, also if there's an established method using the arginine auxtrophic strain,
(ex. the time & concentration of 14C labelled arginine uptake,the ratio of
labelled and non labelled arginine) it will be very helpful.
