Hi Folks,
I am currently working with the "chlamydomonas relative" Dunaliella
tertiolecta. I have been placing the cells under various light and nutrient
stresses and have been watching the appearance of a proteolytic activity.
Last week we had a culture accident in which temperatures rose from 18 to 35
degrees C...and guess what: the proteolytic activity appeared.
I know very little about heat shock proteins, but it strikes me this may be
one. I have protein samples frozen away for western blotting, and am
looking for sources of very small amounts of antibodies (i.e. enough for a
couple assays) to heat shock proteins, e.g. HSP 70, HSP 25, or others you
folks are aware of.
Can anyone help me out?
Cheers.
________________________________________________________________
_/_/_/ _/ _/ _/ Dr. John A. Berges
_/ _/ _/ _/ _/ Oceanic and Atmospheric
_/ _/ _/_/ _/ _/ Sciences Divison
_/_/_/ _/ _/ _/ _/ Brookhaven National Lab
_/ _/ _/ _/ _/ _/ Upton, NY 11973
_/ _/ _/ _/_/ _/ Phone: 516-282-4077
_/_/_/_/ _/ _/ _/_/_/_/ Email: berges at BNLUX1.BNL.GOV
________________________________________________________________
