In trying to optimize transformation of Chlamydomonas, has anybody
tried to use cells that were grown on a L:D regime to see if
harvesting the cells at different times throughout the cycle makes any
difference?
On a related subject, when using a plasmid to transform or cotransform
Chlamydomonas, is it recommended that the plasmid is linearized rather
than supercoiled?
Thankyou.
Roel Funke, formerly "Rose Leviticus"
