transformation

roel funke rfunke at unlinfo.unl.edu
Mon Sep 11 15:53:08 EST 1995


In trying to optimize transformation of Chlamydomonas, has anybody 
tried to use cells that were grown on a L:D regime to see if 
harvesting the cells at different times throughout the cycle makes any
difference?

On a related subject, when using a plasmid to transform or cotransform
Chlamydomonas, is it recommended that the plasmid is linearized rather
than supercoiled?

Thankyou.

Roel Funke, formerly "Rose Leviticus"





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