IUBio

transformation responses

roel funke rfunke at unlinfo.unl.edu
Thu Sep 14 08:12:18 EST 1995


 
Following are some of the responses I got in answer to my question 
about transformation, and whether or not plasmids should be linearized
before using them to transform Chlamydomonas. Thankyou.  

Stefan Fabry (stefan.fabry at rbis1.biologie.uni-regensburg.de) was curious to
hear what got back to me, so I will pass on his angle: 

We are currently also trying some tricks to improve transformation
(low-methylated DNA) and will be curious whether you
got some additional tips from the community.






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