Following are some of the responses I got in answer to my question
about transformation, and whether or not plasmids should be linearized
before using them to transform Chlamydomonas. Thankyou.
Stefan Fabry (stefan.fabry at rbis1.biologie.uni-regensburg.de) was curious to
hear what got back to me, so I will pass on his angle:
We are currently also trying some tricks to improve transformation
(low-methylated DNA) and will be curious whether you
got some additional tips from the community.